CD34 Rabbit Polyclonal Antibody (0108-2)
Catalog# 0108-2
Rabbit Polyclonal to CD34
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Zebrafish
Positive control:
Lane 1: Human brain lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse testis lysate
Lane 4: Mouse thymus lysate
Lane 5: Mouse spleen lysate
Lane 6:Jurkat lysate
Lane 7: TF-1 lysate
Lane 8: F9 lysate
Predicted band size: 41 kDa
Observed band size: 90 kDa
Specifications
Product Type
Rabbit polyclonal primary
Product Name
CD34 Rabbit Polyclonal Antibody (0108-2)
Immunogen
Synthetic peptide within human cd34 aa 336-385.
Host
Rabbit
Positive Control
Human brain, mouse brain, mouse testis, Mouse thymus, mouse spleen, Jurkat, TF-1, F9, human embryonic stem cell, human tonsil tissue
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:100
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IHC-P
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1:3,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD34
SYNONYMS
CD34 antibody;CD34 antigen antibody;CD34 molecule antibody;CD34_HUMAN antibody;Cluster designation 34 antibody;Hematopoietic progenitor cell antigen CD34 antibody;HPCA1 antibody;Mucosialin antibody;OTTHUMP00000034733 antibody;OTTHUMP00000034734 antibody
SEQUENCE SIMILARITIES
Belongs to the CD34 family.
TISSUE SPECIFICITY
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE
On early hematopoietic progenitor cells.
POST-TRANSLATIONAL MODIFICATION
Highly glycosylated.; Phosphorylated on serine residues by PKC.
SUBCELLULAR LOCATION
Cell membrane
FUNCTION
CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. However, little is known about its exact function. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration.
CITATIONS
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Photodynamic therapy of lung cancer with photosensitizers based on polycationic derivatives of synthetic bacteriochlorin (experimental study)
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IHC
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Human
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Mouse
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Citation
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Tetrahedral Framework Nucleic Acids Promote Senile Osteoporotic Fracture Repair by Enhancing Osteogenesis and Angiogenesis of Callus
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WB
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Mouse
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Citation
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