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Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185)) Recombinant Rabbit Monoclonal Antibody [SZ2-4] (ET1603-22)

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Catalog# ET1603-22

Rabbit Monoclonal to Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185))

Tested for

  • WB

  • IF-Cell

  • IHC-P

  • FC

  • IF-Tissue

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185)) Recombinant Rabbit Monoclonal Antibody [SZ2-4] (ET1603-22)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr185 of human erk2.

Host

Rabbit

Modification

Phospho

Modification Site

Erk1(T202)+Erk2(T185)

Positive Control

Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, A549, NIH/3T3, MCF-7, human lung carcinoma tissue, human kidney tissue, human gallbladder tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SZ2-4

RRID

AB_3069673

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100-1:500

  • IHC-P

  • 1:200-1:1,000

  • FC

  • 1:50-1:100

  • IF-Tissue:1:50-1:200

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 43/41 kDa

Isotype

IgG

TARGET

UNIPROT #

P27361

PROTEIN NAME

Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185))

SYNONYMS

ERK 1 antibody;ERK 2 antibody;ERK-1 antibody;ERK-2 antibody;ERK1 antibody;erk1/2 antibody;ERK2 antibody;ERT1 antibody;ERT2 antibody;Extracellular signal regulated kinase 1 antibody;Extracellular signal-regulated kinase 1 antibody;Extracellular signal-regulated kinase 2 antibody;HS44KDAP antibody;HUMKER1A antibody;Insulin-stimulated MAP2 kinase antibody;MAP kinase 1 antibody;MAP kinase 2 antibody;MAP kinase 3 antibody;MAP kinase isoform p42 antibody;MAP kinase isoform p44 antibody;MAPK 1 antibody;MAPK 2 antibody;MAPK 3 antibody;Mapk1 antibody;MAPK2 antibody;MAPK3 antibody;Microtubule-associated protein 2 kinase antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 2 antibody;Mitogen-activated protein kinase 3 antibody;MK01_HUMAN antibody;p38 antibody;p40 antibody;p41 antibody;p41mapk antibody;p42-MAPK antibody;P42MAPK antibody;p44-ERK1 antibody;p44-MAPK antibody;p44ERK1 antibody;p44MAPK antibody;PRKM 2 antibody;PRKM1 antibody;PRKM2 antibody;PRKM3 antibody;protein tyrosine kinase ERK2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Membrane, Cell junction.

FUNCTION

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

CITATIONS

  • Sun Zhijian,et al

    H9N2 Avian Influenza Virus Downregulates FcRY Expression in Chicken Macrophage Cell Line HD11 by Activating the JNK MAPK Pathway

  • Effect of Piper nigrum essential oil in dextran sulfate sodium (DSS)-induced colitis and its potential mechanisms

  • Chen Liangmin,et al

    Psoralen and Isopsoralen, Two Estrogen-Like Natural Products from Psoraleae Fructus, Induced Cholestasis via Activation of ERK1/2

  • Zhang, N., Ma, Q., You, Y., Xia, X., Xie, C., Huang, Y., Wang, Z., Ye, F., Yu, Z., & Xie, X.

    CXCR4-dependent macrophage-to-fibroblast signaling contributes to cardiac diastolic dysfunction in heart failure with preserved ejection fraction

  • Cheng Lin,et al

    Hemodynamic force dictates endothelial angiogenesis through MIEN1-ERK/MAPK-signaling axis

  • Zhang, H., Zhang, W. W., Mo, C. Y., Dong, M. D., Jia, K. T., Liu, W., & Yi, M. S.

    Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fish

  • Xie Binqing,et al

    Cyclic tensile stress promotes osteogenic differentiation via upregulation of Piezo1 in human dental follicle stem cells

  • Identification of a Specific Phage as Growth Factor Alternative Promoting the Recruitment and Differentiation of MSCs in Bone Tissue Regeneration

  • Zhou, S., Ma, Y., Yao, J., Zhao, A., Xie, C., Mi, Y., & Zhang, C.

    TGF-β1-induced collagen promotes chicken ovarian follicle development via an intercellular cooperative pattern

  • J Qian,et al

    The CD163/TWEAK/Fn14 axis: A potential therapeutic target for alleviating inflammatory bone loss

  • Antibacterial and Angiogenic (2A) Bio-Heterojunctions Facilitate Infectious Ischemic Wound Regeneration via an Endogenous-Exogenous Bistimulatory Strategy

  • Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y.

    Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota

  • Lu Py,et al

    Effect and mechanism of recombinant human fibroblast growth factor 18 on osteoporosis in OVX mice

  • wangguiqing

    Astaxanthin protects lipopolysaccharide-induced inflammatory response in Channa argus through inhibiting NF-κB and MAPKs signaling pathways

  • Fang T, Jiang J, Yu W, Li R, Tian H

    DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c-Myc signaling pathway

  • Di Guohui,et al

    Extracellular Vesicles Containing GAS6 Protect the Liver from Ischemia-Reperfusion Injury by Enhancing Macrophage Efferocytosis via MerTK-ERK-COX2 Signaling

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