Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185)) Recombinant Rabbit Monoclonal Antibody [SZ2-4] (ET1603-22)
Catalog# ET1603-22
Rabbit Monoclonal to Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185))
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WB
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IF-Cell
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IHC-P
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FC
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: Jurkat cell lysate
Lane 2: Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 200nM PMA for 30 minutes cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 41/43 kDa
Observed band size: 41/43 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185)) Recombinant Rabbit Monoclonal Antibody [SZ2-4] (ET1603-22)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr185 of human erk2.
Host
Rabbit
Modification
Phospho
Modification Site
Erk1(T202)+Erk2(T185)
Positive Control
Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, A549, NIH/3T3, MCF-7, human lung carcinoma tissue, human kidney tissue, human gallbladder tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SZ2-4
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
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IF-Tissue:1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43/41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Erk1+Erk2 (Erk1(T202)+Erk2(T185))
SYNONYMS
ERK 1 antibody;ERK 2 antibody;ERK-1 antibody;ERK-2 antibody;ERK1 antibody;erk1/2 antibody;ERK2 antibody;ERT1 antibody;ERT2 antibody;Extracellular signal regulated kinase 1 antibody;Extracellular signal-regulated kinase 1 antibody;Extracellular signal-regulated kinase 2 antibody;HS44KDAP antibody;HUMKER1A antibody;Insulin-stimulated MAP2 kinase antibody;MAP kinase 1 antibody;MAP kinase 2 antibody;MAP kinase 3 antibody;MAP kinase isoform p42 antibody;MAP kinase isoform p44 antibody;MAPK 1 antibody;MAPK 2 antibody;MAPK 3 antibody;Mapk1 antibody;MAPK2 antibody;MAPK3 antibody;Microtubule-associated protein 2 kinase antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 2 antibody;Mitogen-activated protein kinase 3 antibody;MK01_HUMAN antibody;p38 antibody;p40 antibody;p41 antibody;p41mapk antibody;p42-MAPK antibody;P42MAPK antibody;p44-ERK1 antibody;p44-MAPK antibody;p44ERK1 antibody;p44MAPK antibody;PRKM 2 antibody;PRKM1 antibody;PRKM2 antibody;PRKM3 antibody;protein tyrosine kinase ERK2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane, Cell junction.
FUNCTION
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.
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