Peroxiredoxin 2 Mouse Monoclonal Antibody [7F2]
Catalog# EM1701-70
Peroxiredoxin 2 Mouse Monoclonal Antibody [7F2]
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WB
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IHC-P
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FC
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IF-Cell
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Human
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unconjugated
Overview
Product Name
Peroxiredoxin 2 Mouse Monoclonal Antibody [7F2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant full length protein of Human PRDX2.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC, IF-Cell
Molecular Weight
Predicted band size: 22 kDa
Positive Control
PC-3M cell lysate, MCF-7 cell lysate, human liver carcinoma tissue, human thyroid carcinoma tissue, human prostate cancer tissue, human kidney tissue, human placenta tissue, Jurkat, PC-3.
Conjugation
unconjugated
Clone Number
7F2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:2000-1:5000
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IHC-P
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1:50-1:1,000
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FC
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1:50-1:200
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IF-Cell
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1:50
Target
Function
The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.
Background References
1. Kang S W et al. Mammalian peroxiredoxin isoforms can reduce hydrogen peroxide generated in response to growth factors and tumor necrosis factor-alpha. J Biol Chem 273:6297-6302 (1998).
2. Kamariah N et al. Transition steps in peroxide reduction and a molecular switch for peroxide robustness of prokaryotic peroxiredoxins. Sci Rep 6:37610-37610 (2016).
Sequence Similarity
Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
Post-translational Modification
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
Epididymis secretory sperm binding protein Li 2a antibody
HEL S 2a antibody
MGC4104 antibody
Natural killer cell enhancing factor B antibody
Natural killer cell-enhancing factor B antibody
Natural Killer Enhancing Factor B antibody
NKEF B antibody
NKEF-B antibody
NKEFB antibody
Peroxiredoxin-2 antibody
ExpandImages
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Western blot analysis of Peroxiredoxin 2 on different lysates with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/5,000 dilution.
Lane 1: PC-3M cell lysate
Lane 2: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 30 seconds;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-70) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-70) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-70) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- Peroxiredoxin 2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti- Peroxiredoxin 2 antibody. Counter stained with hematoxylin.
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Immunocytochemistry analysis of PC-3 cells labeling Peroxiredoxin 2 with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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