Skip to content

Bcl-2 Mouse Monoclonal Antibody [9F1]

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# EM1701-82

Bcl-2 Mouse Monoclonal Antibody [9F1]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Bcl-2 Mouse Monoclonal Antibody [9F1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human BCL2 35-60 aa.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 26 kDa

Positive Control

THP-1 cell lysates, human tonsil tissue.

Conjugation

unconjugated

Clone Number

9F1

RRID

AB_3068806

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:500-1:1,000

Target

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.

Background References

1. Yin X-M et al. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature 369:321-323 (1994).

2. Naumovski L et al. The p53-binding protein 53BP2 also interacts with Bcl2 and impedes cell cycle progression at G2/M. Mol Cell Biol 16:3884-3892 (1996).

Sequence Similarity

Belongs to the Bcl-2 family.

Tissue Specificity

Expressed in a variety of tissues.

Post-translational Modification

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Subcellular Location

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

UNIPROT

SwissProt: P10415 Human

Synonyms

Apoptosis regulator Bcl 2 antibody

Apoptosis regulator Bcl-2 antibody

Apoptosis regulator Bcl2 antibody

AW986256 antibody

B cell CLL/lymphoma 2 antibody

B cell leukemia/lymphoma 2 antibody

Bcl-2 antibody

Bcl2 antibody

BCL2_HUMAN antibody

C430015F12Rik antibody

Expand

Images

  • Western blot analysis of Bcl-2 on THP-1 cell lysates with Mouse anti-Bcl-2 antibody (<a href="/products/EM1701-82" style="font-weight: bold;text-decoration: underline;">EM1701-82</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 1 minute;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-82" style="font-weight: bold;text-decoration: underline;">EM1701-82</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Bcl-2 on THP-1 cell lysates with Mouse anti-Bcl-2 antibody (EM1701-82) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 1 minute;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-82) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BCL2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BCL2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Bcl-2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Mouse Monoclonal Antibody [9F3-R]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2

Application:

Reactivity:

Conjugate:

metafield image

Bcl-2 Mouse Monoclonal Antibody [9F3]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [PD01-41]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,FC

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

FITC Conjugated Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]

Application: FC

Reactivity: Human,Mouse

Conjugate: FITC