IL-6 Mouse Monoclonal Antibody
Catalog# EM30301
IL-6 Mouse Monoclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
IL-6 Mouse Monoclonal Antibody
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL-6 aa 1-211 / 211.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 24 kDa
Positive Control
Jurkat cell lysate, Raji cell lysate, Daudi cell lysate, human tonsil tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
Interleukin 6 (IL-6) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL-6 is a cytokine with a wide variety of biological functions and belongs to the?IL-6 superfamily. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation.
Background References
1. "A nucleotide variant in the promoter region of the interleukin-6 gene associated with decreased bone mineral density."Ota N., Nakajima T., Nakazawa I., Suzuki T., Hosoi T., Orimo H., Inoue S., Shirai Y., Emi M.J. Hum. Genet. 46:267-272(2001)
2. "Association of interleukin-6 promoter variant with bone mineral density in pre-menopausal women."Chung H.W., Seo J.-S., Hur S.E., Kim H.L., Kim J.Y., Jung J.H., Kim L.H., Park B.L., Shin H.D.J. Hum. Genet. 48:243-248(2003)
Sequence Similarity
Belongs to the IL-6 superfamily.
Subcellular Location
Secreted
UNIPROT
Synonyms
Interleukin BSF 2 antibody
B cell differentiation factor antibody
B cell stimulatory factor 2 antibody
B-cell stimulatory factor 2 antibody
BSF 2 antibody
BSF-2 antibody
BSF2 antibody
CDF antibody
CTL differentiation factor antibody
Hepatocyte stimulatory factor antibody
ExpandImages
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Western blot analysis of IL-6 on recombinant human IL-6 protein with Mouse anti-IL-6 antibody (EM30301) at 1/500 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM30301) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
IL-6 Mouse Monoclonal Antibody [1-6]
Application: WB,IF-Cell
Reactivity: Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
IL-6 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated