c-Kit Recombinant Rabbit Monoclonal Antibody [ST04-99] (ET1609-60)

UNIPROT #

P10721

PROTEIN NAME

c-Kit

SYNONYMS

C Kit antibody;c-Kit antibody;c-Kit Ligand antibody;CD117 antibody;Kit antibody;Kit Ligand antibody;KIT oncogene antibody;KIT proto oncogene receptor tyrosine kinase antibody;KIT_HUMAN antibody;Mast cell growth factor receptor antibody;Mast/stem cell growth factor receptor Kit antibody;MGF antibody;p145 c-kit antibody;PBT antibody;Piebald trait protein antibody;Proto oncogene c Kit antibody;Proto oncogene tyrosine protein kinase Kit antibody;Proto-oncogene c-Kit antibody;SCF Receptor antibody;SCFR antibody;soluble KIT variant 1 antibody;Steel Factor Receptor antibody;Stem cell factor receptor antibody;tyrosine protein kinase Kit antibody;Tyrosine-protein kinase Kit antibody;v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody;v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

TISSUE SPECIFICITY

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.; Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.

SUBCELLULAR LOCATION

Cell membrane, Cytoplasm.

FUNCTION

The c-Kit proto-oncogene is a member of the receptor tyrosine kinase family and, more specifically, is closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene (v-Kit), encodes a transmembrane receptor. c-Kit regulates a variety of biological responses including chemotaxis, cell proliferation, apoptosis and adhesion. c-Kit is also identical with the product of the W locus in mice and, as such, is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and is encoded at the murine steel (SI) locus. Kit is the human homolog of the proto-oncogene c-Kit. Mutations in Kit are integral for tumor growth and progression in various cancers.