Glutamine Synthetase Recombinant Rabbit Monoclonal Antibody [PSH10-48]
Catalog# HA723212
Glutamine Synthetase Recombinant Rabbit Monoclonal Antibody [PSH10-48]
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WB
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IHC-Fr
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
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unconjugated
Overview
Product Name
Glutamine Synthetase Recombinant Rabbit Monoclonal Antibody [PSH10-48]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Glutamine Synthetase aa 2-373.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-Fr
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
K-562 cell lysate, HepG2 cell lysate, Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
PSH10-48
Reactivity Data
| WB | IHC-Fr | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
| Cynomolgus Monkey |
|
|
| Pig |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-Fr
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1:500
Target
Function
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
Background References
1. Muthu M. et. al. GLUL Ablation Can Confer Drug Resistance to Cancer Cells via a Malate-Aspartate Shuttle-Mediated Mechanism. Cancers (Basel). 2019 Dec
2. Wang Y. et. al. GLUL Promotes Cell Proliferation in Breast Cancer. J Cell Biochem. 2017 Aug
Subcellular Location
Microsome, Cytosol, Mitochondrion, Cell membrane.
Synonyms
cell proliferation-inducing protein 59 antibody
Cgl2214 antibody
GLNA antibody
GLNA_HUMAN antibody
GLNS antibody
GLUL antibody
Glutamate ammonia ligase antibody
Glutamate decarboxylase antibody
Glutamate--ammonia ligase antibody
glutamine synthase antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Western blot analysis of Glutamine Synthetase on different lysates with Rabbit anti-Glutamine Synthetase antibody (HA723212) at 1/5,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Rat brain tissue lysate
Lane 6: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723212) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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