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Glutamine Synthetase Recombinant Rabbit Monoclonal Antibody [PSH10-48] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751351

Glutamine Synthetase Recombinant Rabbit Monoclonal Antibody [PSH10-48] - BSA and Azide free

Application

  • WB

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

Glutamine Synthetase Recombinant Rabbit Monoclonal Antibody [PSH10-48] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Glutamine Synthetase aa 2-373.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-Fr

Target Molecular Weight

Predicted band size: 42 kDa

Positive Control

K-562 cell lysate, HepG2 cell lysate, Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH10-48

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.

Background References

1. Muthu M. et. al. GLUL Ablation Can Confer Drug Resistance to Cancer Cells via a Malate-Aspartate Shuttle-Mediated Mechanism. Cancers (Basel). 2019 Dec

2. Wang Y. et. al. GLUL Promotes Cell Proliferation in Breast Cancer. J Cell Biochem. 2017 Aug

Subcellular Location

Microsome, Cytosol, Mitochondrion, Cell membrane.

UNIPROT

SwissProt: P15104 Human

SwissProt: P15105 Mouse

SwissProt: P09606 Rat

Synonyms

cell proliferation-inducing protein 59 antibody

Cgl2214 antibody

GLNA antibody

GLNA_HUMAN antibody

GLNS antibody

GLUL antibody

Glutamate ammonia ligase antibody

Glutamate decarboxylase antibody

Glutamate--ammonia ligase antibody

glutamine synthase antibody

Expand

Images

  • This data was developed using <a href="/products/HA723212" style="font-weight: bold;text-decoration: underline;">HA723212</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    This data was developed using HA723212, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA723212" style="font-weight: bold;text-decoration: underline;">HA723212</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    This data was developed using HA723212, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA723212" style="font-weight: bold;text-decoration: underline;">HA723212</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Glutamine Synthetase on different lysates with Rabbit anti-Glutamine Synthetase antibody (<a href="/products/HA723212" style="font-weight: bold;text-decoration: underline;">HA723212</a>) at 1/5,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: Mouse brain tissue lysate<br />Lane 4: Mouse liver tissue lysate<br />Lane 5: Rat brain tissue lysate<br />Lane 6: Rat liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723212" style="font-weight: bold;text-decoration: underline;">HA723212</a>) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723212, the same antibody clone in a different buffer formulation.
    Western blot analysis of Glutamine Synthetase on different lysates with Rabbit anti-Glutamine Synthetase antibody (HA723212) at 1/5,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Mouse brain tissue lysate
    Lane 4: Mouse liver tissue lysate
    Lane 5: Rat brain tissue lysate
    Lane 6: Rat liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723212) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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