iFluor™ 594 Conjugated beta Tubulin Recombinant Mouse Monoclonal Antibody [A1-A4-R]

Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling beta Tubulin (HA600110F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling beta Tubulin (HA600110F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded rat kidney tissue labeling beta Tubulin (HA600110F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Flow cytometric analysis of HeLa cells labeling beta Tubulin.

Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with beta Tubulin (HA600110F, red, 1μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of NIH/3T3 cells labeling beta Tubulin.

Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with beta Tubulin (HA600110F, red, 1μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).