IL-7 Mouse Monoclonal Antibody [A8B11]
Catalog# HA601048
IL-7 Mouse Monoclonal Antibody [A8B11]
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WB
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Human
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unconjugated
Overview
Product Name
IL-7 Mouse Monoclonal Antibody [A8B11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human IL-7 aa 1-177/177.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 20 kDa
Positive Control
HepG2 cell lysates.
Conjugation
unconjugated
Clone Number
A8B11
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500
Target
Function
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL7 plays an essential role in lymphoid cell survival, and in the maintenance of naive and memory T cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be a potent inducer of proinflammatory cytokines and chemokines which may defend against the infection, but may also mediate destructive lung injury. Elevated serum IL7 levels, together with several other circulating cytokines and chemokines, has been found to be associated with the severity of Coronavirus Disease 19 (COVID-19).
Background References
1. Sun S. et. al. Gene fusion of IL7 involved in the regulation of idiopathic pulmonary fibrosis. Ther Adv Respir Dis. 2021 Jan-Dec
2. Perez-Witzke D. et. al. Development of a long-term, IL7 dependent cell death rescue assay in CD4+ T-cells. J Immunol Methods. 2021 Jun
Subcellular Location
Secreted.
UNIPROT
Synonyms
IL 7 antibody
IL-7 antibody
Il7 antibody
IL7_HUMAN antibody
Interleukin 7 antibody
Interleukin-7 antibody
LP-1 antibody
Lymphopoietin 1 antibody
Images
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Western blot analysis of IL-7 on HepG2 cell lysates with Mouse anti-IL-7 antibody (HA601048) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 2 minutes;
15 % SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601048) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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