E-Cadherin Recombinant Mouse Monoclonal Antibody [A0-G11-2-R] (HA601143)
Catalog# HA601143
Mouse Monoclonal to E-Cadherin
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WB
-
IHC
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mIHC
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Human
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Mouse
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Rat
Lane 1: A431 cell lysate
Lane 2: SW480 cell lysate
Lane 3: MCF7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 98 kDa
Observed band size: 130 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601143) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
E-Cadherin Recombinant Mouse Monoclonal Antibody [A0-G11-2-R] (HA601143)
Immunogen
Recombinant protein within mouse e-cadherin aa 350-550.
Host
Mouse
Positive Control
A431 cell lysate, SW480 cell lysate, MCF7 cell lysate, human breast carcinoma tissue, human liver cancer tissue, human liver tissue, human lung cancer tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A0-G11-2-R
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC
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1:200-1:10,000
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mIHC
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1:4,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 98 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
E-Cadherin
SYNONYMS
Cadherin-1;CAM 120/80;Epithelial cadherin (E-cadherin);Uvomorulin
SUBCELLULAR LOCATION
Cell membrane, Endosome, Golgi apparatus.
FUNCTION
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
CITATIONS
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Ming Zhou,et al
Single-Cell Transcriptomic Analysis Reveals Dynamic Cellular Processes in Corneal Epithelium During Wound Healing in Cynomolgus Monkeys
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IF
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Cynomolgus Monkey
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Citation
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