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CGRP Recombinant Antibody [PSH08-45] - Rat IgG1 (Chimeric)

Usd: 315

Specification

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Catalog# HA601423

CGRP Recombinant Antibody [PSH08-45] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CGRP Recombinant Antibody [PSH08-45] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within rat CGRP aa 1-128.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Molecular Weight

Predicted band size: 14 kDa

Positive Control

Mouse colon, mouse spinal cord tissue, rat spinal cord tissue, TT cell lysate.

Conjugation

unconjugated

Clone Number

PSH08-45

Reactivity Data

IHC-Fr IHC-P WB
Human
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Not Recommended
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:2,000

  • WB

  • 1:2,000

Target

Function

Calcitonin gene-related peptide (CGRP) is a member of the calcitonin family of peptides consisting of calcitonin, amylin, adrenomedullin, adrenomedullin 2 (intermedin) and calcitonin‑receptor‑stimulating peptide. CGRP is produced in both peripheral and central neurons. It is a potent peptide vasodilator and can function in the transmission of nociception. In the spinal cord, the function and expression of CGRP may differ depending on the location of synthesis. CGRP is derived mainly from the cell bodies of motor neurons when synthesized in the ventral horn of the spinal cord and may contribute to the regeneration of nervous tissue after injury. Conversely, CGRP is derived from dorsal root ganglion when synthesized in the dorsal horn of the spinal cord and may be linked to the transmission of pain. In the trigeminal vascular system, the cell bodies of the trigeminal ganglion are the main source of CGRP. CGRP is thought to play a role in cardiovascular homeostasis and nociception. In the heart, CGRP acts as a chronotrope by increasing heart rate. Apart from these attributes, CGRP is known to modulate the autonomic nervous system and plays a role in ingestion. CGRP has moderate effects on calcium homeostasis compared to its extensive actions in other areas, such as the autonomic nervous system.

Background References

1. Russo AF et al. CGRP physiology, pharmacology, and therapeutic targets: migraine and beyond. Physiol Rev. 2023 Apr

2. de Vries T et al. Pharmacological treatment of migraine: CGRP and 5-HT beyond the triptans. Pharmacol Ther. 2020 Jul

Subcellular Location

Secreted.

UNIPROT

SwissProt: P06881 Human

SwissProt: P10092 Human

SwissProt: P70160 Mouse

SwissProt: Q99JA0 Mouse

SwissProt: P01256 Rat

SwissProt: P01257 Rat

Synonyms

Calcitonin/calcitonin related polypeptide alpha antibody

Alpha type CGRP antibody

Beta type CGRP antibody

CALC1 antibody

CALC2 antibody

CALCA antibody

CALCB antibody

Calcitonin 1 antibody

Calcitonin 2 antibody

Calcitonin gene related peptide I antibody

Expand

Images

  • Immunofluorescence analysis of frozen mouse colon tissue with Rat anti-CGRP antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span><br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse colon tissue with Rat anti-CGRP antibody (HA601423) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601423, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue with Rat anti-CGRP antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue with Rat anti-CGRP antibody (HA601423) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601423) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rat anti-CGRP antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rat anti-CGRP antibody (HA601423) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601423) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CGRP on different lysates with Rat anti-CGRP antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>) at 1/2,000 dilution.<br /><br />Lane 1: TT cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 10 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601423" style="font-weight: bold;text-decoration: underline;">HA601423</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CGRP on different lysates with Rat anti-CGRP antibody (HA601423) at 1/2,000 dilution.

    Lane 1: TT cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 10 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601423) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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