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GAD65 Recombinant Antibody [PSH06-80] - Chicken IgY (Chimeric)

Usd: 350

Specification

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Catalog# HA601473

GAD65 Recombinant Antibody [PSH06-80] - Chicken IgY (Chimeric)

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

GAD65 Recombinant Antibody [PSH06-80] - Chicken IgY (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 65 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH06-80

Reactivity Data

IHC-Fr WB
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgY

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200

  • WB

  • 1:5,000

Target

Function

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Background References

1. Budhram A et al. Clinical spectrum of high-titre GAD65 antibodies. J Neurol Neurosurg Psychiatry. 2021 Feb

2. Budhram A et al. Positive Predictive Value of Anti-GAD65 ELISA Cut-Offs for Neurological Autoimmunity. Can J Neurol Sci. 2023 Sep

Subcellular Location

Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane.

UNIPROT

SwissProt: P48320 Mouse

SwissProt: Q05683 Rat

Synonyms

65 kDa glutamic acid decarboxylase antibody

DCE 2 antibody

DCE2 antibody

DCE2_HUMAN antibody

GAD 2 antibody

GAD 65 antibody

GAD-2 antibody

GAD-65 antibody

Gad2 antibody

Glutamate decarboxylase 2 (pancreas) antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • Western blot analysis of GAD65 on different lysates with Chicken anti-GAD65 antibody (<a href="/products/HA601473" style="font-weight: bold;text-decoration: underline;">HA601473</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601473" style="font-weight: bold;text-decoration: underline;">HA601473</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (<a href="/products/HA1026" style="font-weight: bold;text-decoration: underline;">HA1026</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GAD65 on different lysates with Chicken anti-GAD65 antibody (HA601473) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601473) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"