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GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric)

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Specification

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Catalog# HA601483

GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

  • WB

  • mIHC

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human GFAP aa 1-50 / 432.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB, mIHC

Molecular Weight

Predicted band size: 50 kDa

Positive Control

Human brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

SA03-04

Reactivity Data

IHC-Fr IHC-P WB mIHC
Mouse
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:1,000

  • IHC-P

  • 1:500

  • WB

  • 1:2,000

  • mIHC

  • 1:1,000

Target

Function

Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is a type III intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS), including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys, Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. GFAP is closely related to the other three non-epithelial type III IF family members, vimentin, desmin and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength as well as the shape of cells, but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

Background References

1. Zhang N et al. A self-assembly peptide nanofibrous scaffold reduces inflammatory response and promotes functional recovery in a mouse model of intracerebral hemorrhage. Nanomedicine N/A:N/A (2016).

2. Green AL et al. Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma. Neuro Oncol 17:697-707 (2015).

Subcellular Location

Cytoplasm

UNIPROT

SwissProt: P03995 Mouse

SwissProt: P47819 Rat

Synonyms

wu:fb34h11 antibody

ALXDRD antibody

cb345 antibody

etID36982.3 antibody

FLJ42474 antibody

FLJ45472 antibody

GFAP antibody

GFAP_HUMAN antibody

gfapl antibody

Glial fibrillary acidic protein antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000 (GFAP, <a href="/products/HA601483" style="font-weight: bold;text-decoration: underline;">HA601483</a>, Rat, red); 1/500 (GAD65, <a href="/products/HA722750" style="font-weight: bold;text-decoration: underline;">HA722750</a>, Rabbit, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/1,000 (GFAP, HA601483, Rat, red); 1/500 (GAD65, HA722750, Rabbit, green)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rat anti-GFAP antibody (<a href="/products/HA601483" style="font-weight: bold;text-decoration: underline;">HA601483</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601483" style="font-weight: bold;text-decoration: underline;">HA601483</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rat anti-GFAP antibody (HA601483) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-GFAP antibody (<a href="/products/HA601483" style="font-weight: bold;text-decoration: underline;">HA601483</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601483" style="font-weight: bold;text-decoration: underline;">HA601483</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-GFAP antibody (HA601483) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of GFAP on different lysates with Rat anti-GFAP antibody (<a href="/products/HA601483" style="font-weight: bold;text-decoration: underline;">HA601483</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br />Lane 3: Mouse liver tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601483" style="font-weight: bold;text-decoration: underline;">HA601483</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of GFAP on different lysates with Rat anti-GFAP antibody (HA601483) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate
    Lane 3: Mouse liver tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601483) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation