Skip to content

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Rat IgG1 (Chimeric)

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# HA601521

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

  • IF-Tissue

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human GLUT1 aa 443-492 / 492.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, IF-Tissue, WB

Molecular Weight

Predicted band size: 54 kDa

Positive Control

Human liver tissue, mouse liver tissue, HeLa cell lysate, HT-29 cell lysate, PC-12 cell lysate, Mouse liver tissue lysate.

Conjugation

unconjugated

Clone Number

SA0377

Reactivity Data

IHC-Fr IHC-P IF-Tissue WB
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:2,000-1:5,000

  • IF-Tissue

  • 1:500-1:1,000

  • WB

  • 1:50,000

Target

Function

Glucose transporter 1 (or GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein that in humans is encoded by the SLC2A1 gene. GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells. This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. One good source of GLUT1 is erythrocyte membranes. GLUT1 accounts for 2 percent of the protein in the plasma membrane of erythrocytes. GLUT1, found in the plasma membrane of erythrocytes, is a classic example of a uniporter. After glucose is transported into the erythrocyte, it is rapidly phosphorylated, forming glucose-6-phosphate, which cannot leave the cell. Mutations in this gene can cause GLUT1 deficiency syndrome 1, GLUT1 deficiency syndrome 2, idiopathic generalized epilepsy 12, dystonia 9, and stomatin-deficient cryohydrocytosis.

Background References

1. Boyer-Di Ponio J et al. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS One 9:e84179 (2014).

2. Saucillo DC et al. Leptin metabolically licenses T cells for activation to link nutrition and immunity. J Immunol 192:136-44 (2014).

Subcellular Location

Cell membrane, Melanosome

UNIPROT

SwissProt: P11166 Human

SwissProt: P17809 Mouse

SwissProt: P11167 Rat

Synonyms

Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody

CSE antibody

DYT17 antibody

DYT18 antibody

DYT9 antibody

EIG12 antibody

erythrocyte/brain antibody

Erythrocyte/hepatoma glucose transporter antibody

facilitated glucose transporter member 1 antibody

Glucose transporter 1 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (<a href="/products/HA601521" style="font-weight: bold;text-decoration: underline;">HA601521</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601521" style="font-weight: bold;text-decoration: underline;">HA601521</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601521) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (<a href="/products/HA601521" style="font-weight: bold;text-decoration: underline;">HA601521</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601521" style="font-weight: bold;text-decoration: underline;">HA601521</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601521) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Glucose Transporter GLUT1 on different lysates with Rat anti-Glucose Transporter GLUT1 antibody (<a href="/products/HA601521" style="font-weight: bold;text-decoration: underline;">HA601521</a>) at 1/50,000 dilution.<br /><br />Lane 1: HeLa cell lysate (no heat)<br />Lane 2: HT-29 cell lysate (no heat)<br />Lane 3: PC-12 cell lysate (no heat)<br />Lane 4: Mouse liver tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 45-60 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601521" style="font-weight: bold;text-decoration: underline;">HA601521</a>) at 1/50,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Glucose Transporter GLUT1 on different lysates with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/50,000 dilution.

    Lane 1: HeLa cell lysate (no heat)
    Lane 2: HT-29 cell lysate (no heat)
    Lane 3: PC-12 cell lysate (no heat)
    Lane 4: Mouse liver tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 45-60 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601521) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application: WB,IHC-Fr,IHC-P,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Mouse IgG1 (Chimeric)

Application: WB,IHC-Fr,IHC-P,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Rat IgG1 (Chimeric) - BSA and Azide free

Application: IHC-Fr,IHC-P,IF-Tissue,WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,IHC-Fr

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,mIHC,IHC-Fr

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

metafield image

Glucose Transporter GLUT1 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

iFluor™ 488 Conjugated Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]

Application: IF-Cell,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: iFluor™ 488

metafield image

iFluor™ 647 Conjugated Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]

Application: IF-Tissue

Reactivity: Human,Mouse

Conjugate: iFluor™ 647