Skip to content

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA601612

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 20 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, C6 (Rat glioma cells) cell lysate, Mouse brain (4weeks) tissue lysate, Rat brain (4weeks) tissue lysate.

Conjugation

unconjugated

Clone Number

PSH23-77

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000-1:5,000

Target

Function

The gene PARK7, also known as DJ-1, encodes a protein of the peptidase C56 family. The human gene PARK7 has 8 exons and locates at chromosome band 1p36.23. DJ-1 was shown to prevent metabolite and protein damage caused by a glycolytic metabolite. This metabolite has been suggested and confirmed to be cyclic 3-phosphoglycerate (or cyclic 3-phosphoglyceric anhydride). Catalytic efficiency of DJ-1 as a hydrolase of cyclic 3-phosphoglyceric anhydride is 10,000 times higher than other reported enzymatic activities of DJ-1. Under an oxidative condition, DJ-1 inhibits the aggregation of α-synuclein via its chaperone activity, thus functioning as a redox-sensitive chaperone and as a sensor for oxidative stress. Accordingly, DJ-1 apparently protects neurons against oxidative stress and cell death. In parallel, protein DJ-1 acts as a positive regulator of androgen receptor-dependent transcription. DJ-1 is expressed in both the neural retina and retinal pigment epithelium of mammals, where it exerts a neuroprotective role against oxidative stress under both physiological and pathological conditions.

Background References

1. Pan J et al. The Imbalance of p53-Park7 Signaling Axis Induces Iron Homeostasis Dysfunction in Doxorubicin-Challenged Cardiomyocytes. Adv Sci (Weinh). 2023 May

2. Qu X et al. PARK7 deficiency inhibits fatty acid beta-oxidation via PTEN to delay liver regeneration after hepatectomy. Clin Transl Med. 2022 Sep

Subcellular Location

Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion, Endoplasmic reticulum.

UNIPROT

SwissProt: Q99497 Human

SwissProt: Q99LX0 Mouse

SwissProt: O88767 Rat

Synonyms

CAP1 antibody

DJ-1 antibody

DJ1 antibody

DJ1 protein antibody

Epididymis secretory sperm binding protein Li 67p antibody

FLJ27376 antibody

FLJ34360 antibody

FLJ92274 antibody

HEL S 67p antibody

Oncogene DJ1 antibody

Expand

Images

  • Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (<a href="/products/HA601612" style="font-weight: bold;text-decoration: underline;">HA601612</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate <br />Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate <br />Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate <br />Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate <br />Lane 5: C6 (Rat glioma cells) cell lysate <br />Lane 6: Mouse brain (4weeks) tissue lysate <br />Lane 7: Rat brain (4weeks) tissue lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 2 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA601612" style="font-weight: bold;text-decoration: underline;">HA601612</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 20 kDa <br />Observed band size: 20 kDa

    Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (HA601612) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
    Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate
    Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate
    Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate
    Lane 5: C6 (Rat glioma cells) cell lysate
    Lane 6: Mouse brain (4weeks) tissue lysate
    Lane 7: Rat brain (4weeks) tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 2 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601612, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601612" style="font-weight: bold;text-decoration: underline;">HA601612</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601612, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601612" style="font-weight: bold;text-decoration: underline;">HA601612</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601612, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601612" style="font-weight: bold;text-decoration: underline;">HA601612</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601612, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

Biotin Conjugated Human PARK7 Recombinant Mouse Monoclonal Antibody [PSH15-68] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: Biotin

metafield image

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77] - BSA and Azide free

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-76]

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-76] - BSA and Azide free

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

HRP Conjugated Human PARK7 Recombinant Mouse Monoclonal Antibody [PSH15-68] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: HRP

metafield image

Human PARK7 Recombinant Mouse Monoclonal Antibody [PSH15-67] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human

Conjugate: unconjugated

metafield image

Human PARK7 Recombinant Mouse Monoclonal Antibody [PSH15-68] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human

Conjugate: unconjugated