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PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610412

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 20 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, C6 (Rat glioma cells) cell lysate, Mouse brain (4weeks) tissue lysate, Rat brain (4weeks) tissue lysate.

Conjugation

unconjugated

Clone Number

PSH23-77

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000-1:5,000

Target

Function

The gene PARK7, also known as DJ-1, encodes a protein of the peptidase C56 family. The human gene PARK7 has 8 exons and locates at chromosome band 1p36.23. DJ-1 was shown to prevent metabolite and protein damage caused by a glycolytic metabolite. This metabolite has been suggested and confirmed to be cyclic 3-phosphoglycerate (or cyclic 3-phosphoglyceric anhydride). Catalytic efficiency of DJ-1 as a hydrolase of cyclic 3-phosphoglyceric anhydride is 10,000 times higher than other reported enzymatic activities of DJ-1. Under an oxidative condition, DJ-1 inhibits the aggregation of α-synuclein via its chaperone activity, thus functioning as a redox-sensitive chaperone and as a sensor for oxidative stress. Accordingly, DJ-1 apparently protects neurons against oxidative stress and cell death. In parallel, protein DJ-1 acts as a positive regulator of androgen receptor-dependent transcription. DJ-1 is expressed in both the neural retina and retinal pigment epithelium of mammals, where it exerts a neuroprotective role against oxidative stress under both physiological and pathological conditions.

Background References

1. Pan J et al. The Imbalance of p53-Park7 Signaling Axis Induces Iron Homeostasis Dysfunction in Doxorubicin-Challenged Cardiomyocytes. Adv Sci (Weinh). 2023 May

2. Qu X et al. PARK7 deficiency inhibits fatty acid beta-oxidation via PTEN to delay liver regeneration after hepatectomy. Clin Transl Med. 2022 Sep

Subcellular Location

Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion, Endoplasmic reticulum.

UNIPROT

SwissProt: Q99497 Human

SwissProt: Q99LX0 Mouse

SwissProt: O88767 Rat

Synonyms

CAP1 antibody

DJ-1 antibody

DJ1 antibody

DJ1 protein antibody

Epididymis secretory sperm binding protein Li 67p antibody

FLJ27376 antibody

FLJ34360 antibody

FLJ92274 antibody

HEL S 67p antibody

Oncogene DJ1 antibody

Expand

Images

  • Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (<a href="/products/HA610412" style="font-weight: bold;text-decoration: underline;">HA610412</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate <br />Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate <br />Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate <br />Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate <br />Lane 5: C6 (Rat glioma cells) cell lysate <br />Lane 6: Mouse brain (4weeks) tissue lysate <br />Lane 7: Rat brain (4weeks) tissue lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 2 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA610412" style="font-weight: bold;text-decoration: underline;">HA610412</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 20 kDa <br />Observed band size: 20 kDa

    Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (HA610412) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
    Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate
    Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate
    Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate
    Lane 5: C6 (Rat glioma cells) cell lysate
    Lane 6: Mouse brain (4weeks) tissue lysate
    Lane 7: Rat brain (4weeks) tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 2 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA610412, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610412" style="font-weight: bold;text-decoration: underline;">HA610412</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610412, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610412" style="font-weight: bold;text-decoration: underline;">HA610412</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610412, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610412" style="font-weight: bold;text-decoration: underline;">HA610412</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610412, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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