CD4 Recombinant Antibody [ST0488] - Rat IgG1 (Chimeric)
Catalog# HA601671
CD4 Recombinant Antibody [ST0488] - Rat IgG1 (Chimeric)
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WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
CD4 Recombinant Antibody [ST0488] - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Human CD4 aa 196-416 / 458.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
THP-1 (Human acute monoblastic leukemia cell) cell lysate, U-937 (Human acute monocytic leukemia cell) cell lysate.
Conjugation
unconjugated
Clone Number
ST0488
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:500-1:1,000
Target
Function
The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8; T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4 (also designated T4 and Leu 3), is a membrane glycoprotein that contains four extracellular immunoglobin-like domains. The TCR in association with CD4 can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex. An additional role of CD4 is to function as a receptor for HIV.
Background References
1. Kim EJ et al. Costimulation blockade alters germinal center responses and prevents antibody-mediated rejection. Am J Transplant 14:59-69 (2014).
2. Liu XD et al. Resistance to Antiangiogenic Therapy Is Associated with an Immunosuppressive Tumor Microenvironment in Metastatic Renal Cell Carcinoma. Cancer Immunol Res 3:1017-29 (2015).
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 4 antibody
CD4 (L3T4) antibody
CD4 antibody
CD4 antigen (p55) antibody
CD4 antigen antibody
CD4 molecule antibody
CD4 receptor antibody
CD4+ Lymphocyte deficiency, included antibody
CD4_HUMAN antibody
CD4mut antibody
ExpandImages
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Western blot analysis of CD4 on different lysates with Rat anti-CD4 antibody (HA601671) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 2: U-937 (Human acute monocytic leukemia cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute; ECL: K1803
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601671, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 51 kDa
Observed band size: 55 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601671, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lymph nodes
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601671, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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