alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610267
alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric) - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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FC(Intra)
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within N-terminal human alpha smooth muscle Actin.
Product Specificity
Rat secondary antibodies are required for detection. This product has cross-reactions with rabbit secondary antibodies.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC(Intra)
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
Saos-2 (Human osteosarcoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C2C12 (Mouse myoblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, Mouse skin tissue lysate, Rat skin tissue lysate.
Conjugation
unconjugated
Clone Number
SY02-64
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:5,000
-
IHC-P
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1:50,000
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FC(Intra)
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1:1,000
Target
Function
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
Background References
1. Izumi D et al. CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin 1 clustering and invasiveness in gastric cancer. Int J Cancer 138:1207-19 (2016).
2. Chung SI et al. Development of a transgenic mouse model of hepatocellular carcinoma with a liver fibrosis background. BMC Gastroenterol 16:13 (2016).
Sequence Similarity
Belongs to the actin family.
Post-translational Modification
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.; Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-75 by SETD3.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Subcellular Location
Cytoplasm.
Synonyms
alpha SMA
a-SMA antibody
asma antibody
a actin antibody
AAT6 antibody
ACTA_HUMAN antibody
ACTA2 antibody
Actin alpha 2 smooth muscle aorta antibody
Actin aortic smooth muscle antibody
Actin, aortic smooth muscle antibody
ExpandImages
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Western blot analysis of alpha smooth muscle Actin on different lysates with Rat anti-alpha smooth muscle Actin antibody (HA610267) at 1/5,000 dilution.
Lane 1: Saos-2 (Human osteosarcoma cell) cell lysate
Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: C2C12 (Mouse myoblast) cell lysate
Lane 5: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lane 6: Mouse skin tissue lysate
Lane 7: Rat skin tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610267, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA610267, 1/5,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Small intestine
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610267, 1/50,000, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Human
Sample: Saos-2 (Human osteosarcoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA610267 (1/1,000, Red), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 15 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA610267 (1/1,000, Red), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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