Mesothelin Recombinant Rabbit Monoclonal Antibody [PD00-68]

☑ Relative expression (RE)

Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (HA721175) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: SK-OV-3 cell lysate
Lane 3: OVCAR-3 cell lysate
Lane 4: SiHa cell lysate
Lane 5: NCI-H226 cell lysate
Lane 6: PC-3M cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 45 kDa

Exposure time: 7 seconds;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721175) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Application: IF-Tissue

Species: Human

Site: ovarian cancer

Sample: Paraffin-embedded section

Antibody concentration: 1/500

Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue with Rabbit anti-Mesothelin antibody (HA721175) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721175) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue with Rabbit anti-Mesothelin antibody (HA721175) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721175) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Mesothelin antibody (HA721175) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721175) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.