Human IL-10 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Detector) [PS00-81] (HA721237)
Catalog# HA721237
Rabbit Monoclonal to Human IL-10
Tested for
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ELISA(Det)
Reacts with
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Human
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Dog
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Cynomolgus monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Sandwich ELISA analysis of IL-10 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721236 [PS00-98] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-10 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721237 [PS00-81] (Biotin, 1:2000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-10 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.