CD55 Recombinant Rabbit Monoclonal Antibody [JE31-59]
Catalog# HA722724
CD55 Recombinant Rabbit Monoclonal Antibody [JE31-59]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
CD55 Recombinant Rabbit Monoclonal Antibody [JE31-59]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CD55 aa 101-150 / 381.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 41 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, K-562 cell lysate, HT-29 cell lysate, human lung tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
JE31-59
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
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IHC-P
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1:200
Target
Function
Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. DAF regulates the complement system on the cell surface. It recognizes C4b and C3b fragments that are created during activation of C4 (classical or lectin pathway) or C3 (alternative pathway). Interaction of DAF with cell-associated C4b of the classical and lectin pathways interferes with the conversion of C2 to C2b, thereby preventing formation of the C4b2a C3-convertase, and interaction of DAF with C3b of the alternative pathway interferes with the conversion of factor B to Bb by factor D, thereby preventing formation of the C3bBb C3 convertase of the alternative pathway. Thus, by limiting the amplification convertases of the complement cascade, DAF indirectly blocks the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system.
Background References
1. Bharti R et al. CD55 in cancer: Complementing functions in a non-canonical manner. Cancer Lett. 2022 Dec
2. Shao F et al. Silencing EGFR-upregulated expression of CD55 and CD59 activates the complement system and sensitizes lung cancer to checkpoint blockade. Nat Cancer. 2022 Oct
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 55 antibody
CD55 antibody
CD55 antigen antibody
CD55 Cromer blood group system antibody
CD55 molecule (Cromer blood group) antibody
CD55 molecule antibody
CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
Cd55a antibody
Complement decay accelerating factor antibody
Complement decay-accelerating factor antibody
ExpandImages
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Western blot analysis of CD55 on different lysates with Rabbit anti-CD55 antibody (HA722724) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 70-75 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722724) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CD55 on different lysates with Rabbit anti-CD55 antibody (HA722724) at 1/10,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD55 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 70-75 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722724) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CD55 antibody (HA722724) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722724) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD55 antibody (HA722724) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722724) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD55 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P
Reactivity: Human
Conjugate: unconjugated
