Human IL-13R alpha 2 Recombinant Rabbit Monoclonal Antibody [PSH07-64] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Human IL-13 R alpha 2 matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722880) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rrecombinant Human IL-13R alpha 2 protein (HA210969) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA722881, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native human IL-13 R alpha 2 in U-87 MG extract samples based on a 1,000 µg/ml extract load.

The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 50%. The mean human IL-13 R alpha 2 concentration was determined to be 4,569 pg/ml in U-87 MG extract.

Interpolated concentrations of native human IL-13 R alpha 2 in A375 extract samples based on a 1,000 µg/ml extract load.

The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are A375 extract 10%. The mean human IL-13 R alpha 2 concentration was determined to be 123.1 ng/ml in A375 extract.

Interpolated concentrations of spiked IL-13 R alpha 2 in human cell culture media samples.

The concentrations of IL-13 R alpha 2 were interpolated from the IL-13 R alpha 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.