Human Chromogranin A Recombinant Rabbit Monoclonal Antibody [PSH28-52] - BSA and Azide free (Capture)

Sandwich ELISA analysis of Human Chromogranin A protein matched pair antibodies

Capture: HA724790, Human Chromogranin A Rabbit mAb [PSH28-52]
Detector: HA724791, Human Chromogranin A Rabbit mAb [PSH28-53]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724790) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Chromogranin A protein (HA211470) starting from 8,000 pg/mL to 0 pg/mL and detect antibody (HA724791, Biotin, 0.1 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Chromogranin A in SH-SY5Y and IMR-32 cell extract samples based on a 1,000 µg/mL extract load.

Capture: HA724790, Human Chromogranin A Rabbit mAb [PSH28-52]
Detector: HA724791, Human Chromogranin A Rabbit mAb [PSH28-53]

The concentrations of Chromogranin A were measured in duplicates, interpolated from the Chromogranin A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Chromogranin A concentration was determined to be 72,241 pg/mL in SH-SY5Y cell extract and 1,884 pg/mL in IMR-32 cell extract.

Interpolated concentrations of native Chromogranin A in human serum samples.

Capture: HA724790, Human Chromogranin A Rabbit mAb [PSH28-52]
Detector: HA724791, Human Chromogranin A Rabbit mAb [PSH28-53]

The concentrations of Chromogranin A were measured in duplicates, interpolated from the Chromogranin A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Chromogranin A concentration was determined to be 22.3 ng/mL in human serum.

Interpolated concentrations of spiked Chromogranin A in human cell culture media samples.

Capture: HA724790, Human Chromogranin A Rabbit mAb [PSH28-52]
Detector: HA724791, Human Chromogranin A Rabbit mAb [PSH28-53]

The concentrations of Chromogranin A were measured in duplicates, interpolated from the Chromogranin A standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).