Biotin Conjugated Human IL-10RB Recombinant Rabbit Monoclonal Antibody [PSH17-45] - Detector

Sandwich ELISA analysis of Human IL-10RB matched pair antibodies

Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44]
Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45]

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA725295 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-10RB (HA211205) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725296, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-10RB in THP-1 cell extract:

Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44]
Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45]

The concentrations of IL-10RB were measured in duplicates, interpolated from the IL-10RB standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10RB concentration was determined to be 861.6 pg/ml in THP-1 cell extract.