PSD95 Recombinant Rabbit Monoclonal Antibody [SR38-09] - BSA and Azide free
Catalog# HA750035
PSD95 Recombinant Rabbit Monoclonal Antibody [SR38-09] - BSA and Azide free
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WB
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IF-Tissue
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IHC-P
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IHC-Fr
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IP
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VIVIT
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Human
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Mouse
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Rat
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ET1602-20
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
PSD95 Recombinant Rabbit Monoclonal Antibody [SR38-09] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PSD95 aa 1-40.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IF-Tissue, IHC-P, IHC-Fr, IP, VIVIT
Molecular Weight
Predicted band size: 80 kDa
Positive Control
Human brain tissue lysate, mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse brain tissue, mouse retina tissue, rat brain tissue, rat retina tissue, mouse cerebellum tissue.
Conjugation
unconjugated
Clone Number
SR38-09
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Tissue
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1:500
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IHC-P
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1:2,000
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IHC-Fr
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1:500-1:1,000
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IP
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1-2μg/sample
Target
Function
PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (discs large homolog 4) gene. PSD-95 is a member of the membrane-associated guanylate kinase (MAGUK) family. With PSD-93 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. PSD-95 is the best studied member of the MAGUK-family of PDZ domain-containing proteins. Like all MAGUK-family proteins, its basic structure includes three PDZ domains, an SH3 domain, and a guanylate kinase-like domain (GK) connected by disordered linker regions. It is almost exclusively located in the post synaptic density of neurons, and is involved in anchoring synaptic proteins. Its direct and indirect binding partners include neuroligin, NMDA receptors, AMPA receptors, and potassium channels. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation.
Background References
1. Fred SM et al. TRKB interaction with PSD95 is associated with latency of fluoxetine and 2R,6R-hydroxynorketamine. Eur J Neurosci. 2023 Apr
2. Yang L et al. Targeting PSD95/nNOS by ZL006 alleviates social isolation-induced heightened attack behavior in mice. Psychopharmacology (Berl). 2022 Jan
Sequence Similarity
Belongs to the MAGUK family.
Tissue Specificity
Brain.
Post-translational Modification
Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses (By similarity). Palmitoylation by ZDHHC2 occurs when the synaptic activity decreases and induces DLG4 synaptic clustering (By similarity). Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering (By similarity). Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycles during neuronal development which slow down in mature neurons (By similarity).; Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis.
Subcellular Location
Cell membrane, Postsynaptic density, Synapse, Cytoplasm, Cell projection, axon, dendritic spine, dendrite, Presynapse.
Synonyms
Discs large homolog 4 antibody
Disks large homolog 4 antibody
DLG 4 antibody
Dlg4 antibody
DLG4_HUMAN antibody
FLJ97752 antibody
FLJ98574 antibody
Human post synaptic density protein 95 antibody
Post synaptic density protein 95 antibody
Postsynaptic density protein 95 antibody
ExpandImages
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Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody (HA750035) at 1/2,000 dilution.
Lane 1: Human brain tissue lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (20 µg/Lane)
Lane 3: Mouse hippocampus tissue lysate (20 µg/Lane)
Lane 4: Rat brain tissue lysate (20 µg/Lane)
Lane 5: Rat hippocampus tissue lysate (20 µg/Lane)
Predicted band size: 80 kDa
Observed band size: 75-100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750035) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PSD95 antibody (HA750035) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750035) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PSD95 antibody (HA750035) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750035) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PSD95 antibody (HA750035) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750035) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-PSD95 antibody (HA750035) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750035) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSD95 antibody (HA750035) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750035) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-PSD95 antibody (HA750035) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750035) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunoprecipitation (IP)
PSD95 was immunoprecipitated in 0.2 mg Mouse brain tissue lysate with HA750035 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750035 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA750035 IP in Mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA750035 in Mouse brain tissue lysate
Exposure time: 14 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA750035, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 80 kDa
Observed band size: 75-100 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Conjugate: unconjugated
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