EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69] - BSA and Azide free
Catalog# HA750703
EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69] - BSA and Azide free
-
IHC-P
-
IF-Cell
-
FC
-
Human
-
HA721644
含抗保成分
-
unconjugated
Overview
Product Name
EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human EpCAM aa1-295.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Cell, FC
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Human colon tissue, HT-29.
Conjugation
unconjugated
Clone Number
PS01-69
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
-
1:200
-
IF-Cell
-
1:100
-
FC
-
1:500-1:1,000
Target
Function
EPCAM is a carcinoma-associated antigen and belongs to a family which includes at least 2 type I membrane proteins. The EPCAM protein has a role in embryonic stem cells proliferation and differentiation. EPCAM is used as a target for immunotherapy treatment of human carcinomas. EPCAM is expressed on most normal epithelial cells and gastrointestinal carcinomas and acts as a homotypic calcium-independent cell adhesion molecule. Epithelial cell adhesion molecules (EPCAM) can act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for supplying immunological barrier as a first line of defense against mucosal infection. EPCAM gene mutations result in congenital tufting enteropathy.
Background References
1. Li D et al. EpCAM-targeting CAR-T cell immunotherapy is safe and efficacious for epithelial tumors. Sci Adv. 2023 Dec
2. Li F et al. EpCAM-Targeting Aptamer Radiotracer for Tumor-Specific PET Imaging. Bioconjug Chem. 2021 Jun
Sequence Similarity
Belongs to the EPCAM family.
Tissue Specificity
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Post-translational Modification
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Subcellular Location
Lateral cell membrane, Cell junction.
UNIPROT
Synonyms
17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody
AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
ExpandImages
-
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-EpCAM antibody (HA750703) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750703) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HT-29 cells labeling EpCAM with Rabbit anti-EpCAM antibody (HA750703) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EpCAM antibody (HA750703) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HT-29 cells labeling EpCAM.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA750703, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
EpCAM Mouse Monoclonal Antibody [7E11]
Application: WB,IHC-P,ELISA
Reactivity: Human
Conjugate: unconjugated
EpCAM Recombinant Rabbit Monoclonal Antibody
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
EpCAM Mouse Monoclonal Antibody [3-E1-E2]
Application: WB,IF-Cell
Reactivity: Human,Mouse
Conjugate: unconjugated
EpCAM Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
iFluor™ 555 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
Application: FC
Reactivity: Human
Conjugate: iFluor™ 555
iFluor™ 647 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
Application: FC,IF-Cell
Reactivity: Human
Conjugate: iFluor™ 647
EpCAM Recombinant Rabbit Monoclonal Antibody [SU03-32]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
Application: IHC-P,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
