Mammalian GFAP DTSet enzyme-linked immunoassay kit
Catalog# DSEH017805；DSEH017815
Mammalian GFAP DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mammalian
Overview
Product Name
Mammalian GFAP DTSet enzyme-linked immunoassay kit
Product Type
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0382 | 0.0432 | 0.0407 | |
| 312.50 | 0.1370 | 0.1330 | 0.1350 | 0.0943 |
| 625.00 | 0.2351 | 0.2153 | 0.2252 | 0.1845 |
| 1250.00 | 0.3858 | 0.3999 | 0.3929 | 0.3522 |
| 2500.00 | 0.7650 | 0.7750 | 0.7700 | 0.7293 |
| 5000.00 | 1.5470 | 1.5810 | 1.5640 | 1.5233 |
| 10000.00 | 2.9470 | 2.8550 | 2.9010 | 2.8603 |
| 20000.00 | 4.3648 | 4.5576 | 4.4612 | 4.4205 |
Experiment Type
Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)
Species Reactivity
Mammalian
Detection Method
Colorimetric Method
Experiment Detection Duration
120min
Standard Curve Range
312.5 pg/ml -20000 pg/ml
Standard Curve Gradient
7 Points/3 Folds
Number of Incubations
2
Detectable Sample
Sample Loading Volume
50 μl
Product Features
- Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
- A development protocol is provided to guide further analysis optimization.
- Detection can be customized according to your specific needs.
- An economical alternative to a complete kit.
Kit Components
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin-HRP Conjugate
Other Required Materials
DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.
The components listed above can be purchased separately.
- 96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
- Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
- Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
- Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
Target
UNIPROT
Synonyms
wu:fb34h11
ALXDRD
cb345
etID36982.3
FLJ42474
FLJ45472
GFAP
GFAP_HUMAN
gfapl
Glial fibrillary acidic protein
ExpandFunction
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.
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