Mammalian VEGF-C DTSet enzyme-linked immunoassay kit
Catalog# DSEH011305；DSEH011315
Mammalian VEGF-C DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mammalian
Overview
Product Name
Mammalian VEGF-C DTSet enzyme-linked immunoassay kit
Product Type
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0589 | 0.0597 | 0.0593 | |
| 78.13 | 0.1390 | 0.1410 | 0.1400 | 0.0807 |
| 156.25 | 0.1787 | 0.1760 | 0.1774 | 0.1181 |
| 312.50 | 0.3264 | 0.3118 | 0.3191 | 0.2598 |
| 625.00 | 0.5674 | 0.5611 | 0.5643 | 0.5050 |
| 1250.00 | 0.9448 | 0.9407 | 0.9428 | 0.8835 |
| 2500.00 | 1.6510 | 1.7680 | 1.7095 | 1.6502 |
| 5000.00 | 3.9570 | 4.1285 | 4.0428 | 3.9835 |
Experiment Type
Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)
Species Reactivity
Mammalian
Detection Method
Colorimetric Method
Experiment Detection Duration
120min
Standard Curve Range
78.13pg/ml-5000pg/ml
Standard Curve Gradient
7 Points/3 Folds
Number of Incubations
2
Detectable Sample
Sample Loading Volume
50 μl
Product Features
- Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
- A development protocol is provided to guide further analysis optimization.
- Detection can be customized according to your specific needs.
- An economical alternative to a complete kit.
Kit Components
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin-HRP Conjugate
Other Required Materials
DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.
The components listed above can be purchased separately.
- 96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
- Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
- Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
- Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
Target
UNIPROT
Synonyms
Flt 4L
Flt4 ligand
FLT4 ligand DHM
Flt4-L
Flt4L
Vascular endothelial growth factor C
Vascular endothelial growth factor related protein
Vascular endothelial growth factor-related protein
VEGF C
VEGF-C
ExpandFunction
Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.
Same Target and Pathway Products
Mammalian TGF beta1 enzyme-linked immunoassay kit
Application: ELISA
Reactivity: Mammalian
Conjugate:
Mammalian GFAP DTSet enzyme-linked immunoassay kit
Application: ELISA
Reactivity: Mammalian
Conjugate:
Mammalian VEGF-C DTSet enzyme-linked immunoassay kit
Application: ELISA
Reactivity: Mammalian
Conjugate:
Mammalian Insulin DTSet enzyme-linked immunoassay kit
Application: ELISA
Reactivity: Mammalian
Conjugate:
