Mammalian Insulin DTSet enzyme-linked immunoassay kit
Catalog# DSEH003805；DSEH003815
Mammalian Insulin DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mammalian
Overview
Product Name
Mammalian Insulin DTSet enzyme-linked immunoassay kit
Product Type
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0083 | 0.0085 | 0.0084 | |
| 312.50 | 0.0297 | 0.0331 | 0.0314 | 0.0230 |
| 625.00 | 0.0839 | 0.0953 | 0.0896 | 0.0812 |
| 1250.00 | 0.2674 | 0.2932 | 0.2803 | 0.2719 |
| 2500.00 | 0.8188 | 0.8938 | 0.8563 | 0.8479 |
| 5000.00 | 2.0540 | 2.0150 | 2.0345 | 2.0261 |
| 10000.00 | 3.4390 | 3.4410 | 3.4400 | 3.4316 |
| 20000.00 | 4.2586 | 4.2479 | 4.2533 | 4.2449 |
Experiment Type
Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)
Species Reactivity
Mammalian
Detection Method
Colorimetric Method
Experiment Detection Duration
120min
Standard Curve Range
312.5pg/ml-20000pg/ml
Standard Curve Gradient
7 Points/3 Folds
Number of Incubations
2
Detectable Sample
Sample Loading Volume
50 μl
Product Features
- Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
- A development protocol is provided to guide further analysis optimization.
- Detection can be customized according to your specific needs.
- An economical alternative to a complete kit.
Kit Components
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin-HRP Conjugate
Other Required Materials
DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.
The components listed above can be purchased separately.
- 96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
- Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
- Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
- Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
Target
UNIPROT
Synonyms
Function
Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.
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