Human/Monkey/Canine IL-12B/IL-12 p40 enzyme-linked immunoassay kit
Catalog# EH0098
Human/Monkey/Canine IL-12B/IL-12 p40 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
1.37~1000 pg/ml
Overview
Product Name
Human/Monkey/Canine IL-12B/IL-12 p40 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.29 pg/ml (50 μl)；1.84 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0228 | 0.0235 | 0.0232 | |
| 1.37 | 0.0310 | 0.0304 | 0.0307 | 0.0076 |
| 4.12 | 0.0481 | 0.0481 | 0.0481 | 0.0250 |
| 12.35 | 0.0945 | 0.0931 | 0.0938 | 0.0707 |
| 37.04 | 0.2360 | 0.2307 | 0.2334 | 0.2102 |
| 111.11 | 0.6390 | 0.6322 | 0.6356 | 0.6125 |
| 333.33 | 1.7120 | 1.6570 | 1.6845 | 1.6614 |
| 1000.00 | 3.8910 | 3.8380 | 3.8645 | 3.8414 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.4 | 104.0 | 339.2 | 19.3 | 101.1 | 309.6 |
| Standard Deviation | 0.7 | 4.3 | 14.4 | 1.3 | 5.4 | 20.1 |
| Coefficient of Variation (%) | 3.4 | 4.1 | 4.2 | 6.6 | 5.4 | 6.5 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 25.15-193.73 | 100 | 80.27 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃