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Human IL-12B/IL-12 p40 enzyme-linked immunoassay kit (EH0098)

Price: Regular price $499.00

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Catalog# EH0098

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.29 pg/ml (50 μl)；1.84 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02280.02350.0232
1.370.03100.03040.03070.0076
4.120.04810.04810.04810.0250
12.350.09450.09310.09380.0707
37.040.23600.23070.23340.2102
111.110.63900.63220.63560.6125
333.331.71201.65701.68451.6614
1000.003.89103.83803.86453.8414

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)19.4104.0339.219.3101.1309.6
Standard Deviation0.74.314.41.35.420.1
Coefficient of Variation(%)3.44.14.26.65.46.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3025.15-193.7310080.27

Sensitivity

0.29 pg/ml (50 μl)；1.84 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-12B p40 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 90% to 117% with an overall mean recovery of 102%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-12B/IL-12 p40 enzyme-linked immunoassay kit (EH0098)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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