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Histone H3 (acetyl K18)
Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

DATA SHEET
MSDS
COA

Usd: 350

Specification

100μl

Quantity

Bulk Order Quote | Customization Request

Catalog# HA600091

Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

Application

WB
IF-Cell

Reactivity

Human

Conjugation

unconjugated

Overview

Product Name

Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Histone H3 aa 1-50 / 136.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 15 kDa

Positive Control

Hela treated with TSA cell lysate, Hela, TSA treated Hela.

Conjugation

unconjugated

Clone Number

A7B6

RRID

AB_3071707

Product Features

Form

Liquid

Concentration

Lot Concentration Lookup

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

WB
1:2,000

IF-Cell
1:200

Target

Function

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Background References

1. Benard A et al. Nuclear expression of histone deacetylases and their histone modifications predicts clinical outcome in colorectal cancer. Histopathology 66:270-82 (2015).

2. Zhang J et al. SOX4 inhibits GBM cell growth and induces G0/G1 cell cycle arrest through Akt-p53 axis. BMC Neurol 14:207 (2014).

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: P68431 Human

SwissProt: P84243 Human

SwissProt: Q16695 Human

SwissProt: Q6NXT2 Human

SwissProt: Q71DI3 Human

Synonyms

H3.3A antibody

HIST1 cluster, H3E antibody

H3 histone family, member A antibody

H3.1 antibody

H3/l antibody

H3F3 antibody

H3FF antibody

H3FJ antibody

H3FL antibody

Histone gene cluster 1, H3 histone family, member E antibody

Expand

H3.3A antibody

HIST1 cluster, H3E antibody

H3 histone family, member A antibody

H3.1 antibody

H3/l antibody

H3F3 antibody

H3FF antibody

H3FJ antibody

H3FL antibody

Histone gene cluster 1, H3 histone family, member E antibody

histone H3.1t antibody

Histone H3/o antibody

FLJ92264 antibody

H 3 antibody

H3 antibody

H3 histone family, member B antibody

H3 histone family, member C antibody

H3 histone family, member D antibody

H3 histone family, member F antibody

H3 histone family, member H antibody

H3 histone family, member I antibody

H3 histone family, member J antibody

H3 histone family, member K antibody

H3 histone family, member L antibody

H3 histone family, member T antibody

H3 histone, family 3A antibody

H3/A antibody

H3/b antibody

H3/c antibody

H3/d antibody

h3/f antibody

H3/h antibody

H3/i antibody

H3/j antibody

H3/k antibody

H3/t antibody

H31_HUMAN antibody

H3F1K antibody

H3F3A antibody

H3FA antibody

H3FB antibody

H3FC antibody

H3FD antibody

H3FH antibody

H3FI antibody

H3FK antibody

HIST1 cluster, H3A antibody

HIST1 cluster, H3B antibody

HIST1 cluster, H3C antibody

HIST1 cluster, H3D antibody

HIST1 cluster, H3F antibody

HIST1 cluster, H3G antibody

HIST1 cluster, H3H antibody

HIST1 cluster, H3I antibody

HIST1 cluster, H3J antibody

HIST1H3A antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

HIST1H3E antibody

HIST1H3F antibody

HIST1H3G antibody

HIST1H3H antibody

HIST1H3I antibody

HIST1H3J antibody

HIST3H3 antibody

Histone 1, H3a antibody

Histone 1, H3b antibody

Histone 1, H3c antibody

Histone 1, H3d antibody

Histone 1, H3e antibody

Histone 1, H3f antibody

Histone 1, H3g antibody

Histone 1, H3h antibody

Histone 1, H3i antibody

Histone 3, H3 antibody

histone cluster 1 H3 family member a antibody

histone cluster 1 H3 family member b antibody

histone cluster 1 H3 family member c antibody

histone cluster 1 H3 family member d antibody

histone cluster 1 H3 family member e antibody

histone cluster 1 H3 family member f antibody

histone cluster 1 H3 family member g antibody

histone cluster 1 H3 family member h antibody

histone cluster 1 H3 family member i antibody

histone cluster 1 H3 family member j antibody

Histone cluster 1, H3a antibody

Histone cluster 1, H3b antibody

Histone cluster 1, H3c antibody

Histone cluster 1, H3d antibody

Histone cluster 1, H3e antibody

Histone cluster 1, H3f antibody

Histone cluster 1, H3g antibody

Histone cluster 1, H3i antibody

Histone cluster 1, H3j antibody

Histone gene cluster 1, H3 histone family, member A antibody

Histone gene cluster 1, H3 histone family, member B antibody

Histone gene cluster 1, H3 histone family, member C antibody

Histone gene cluster 1, H3 histone family, member D antibody

Histone gene cluster 1, H3 histone family, member F antibody

Histone gene cluster 1, H3 histone family, member G antibody

Histone gene cluster 1, H3 histone family, member H antibody

Histone gene cluster 1, H3 histone family, member I antibody

Histone gene cluster 1, H3 histone family, member J antibody

Histone gene cluster 1, H3A antibody

Histone gene cluster 1, H3B antibody

Histone gene cluster 1, H3C antibody

Histone gene cluster 1, H3D antibody

Histone gene cluster 1, H3E antibody

Histone gene cluster 1, H3F antibody

Histone gene cluster 1, H3G antibody

Histone gene cluster 1, H3H antibody

Histone gene cluster 1, H3I antibody

Histone gene cluster 1, H3J antibody

Histone H 3 antibody

Histone H3.1 antibody

Histone H3.2 antibody

Histone H3.3 antibody

Histone H3/a antibody

Histone H3/b antibody

Histone H3/c antibody

Histone H3/d antibody

Histone H3/f antibody

Histone H3/h antibody

Histone H3/i antibody

Histone H3/j antibody

Histone H3/k antibody

Histone H3/l antibody

Histone H3/m antibody

H3K18ac antibody

Collapse

Images

☑ Cell treatment (CT)

Western blot analysis of Histone H3 (acetyl K18) on different lysates with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/2,000 dilution.

Lane 1: Hela cell lysate
Lane 2: Hela treated with TSA cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 14 kDa

Exposure time: 30 seconds;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of TSA treated Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

Usd: 350

Specification

Overview

Product Name

Antibody Type

Immunogen

Species Reactivity

Validated Applications

Molecular Weight

Positive Control

Conjugation

Clone Number

RRID

Product Features

Form

Concentration

Storage Instructions

Storage Buffer

Isotype

Purification Method

Application Dilution

Target

Function

Background References

Subcellular Location

UNIPROT

Synonyms

Images

Products with the same target and pathway

Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427]

Application: WB,IF-Cell,IF-Tissue,IHC-P,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 Rabbit Polyclonal Antibody

Application: WB

Reactivity: Zebrafish,Human,Mouse

Conjugate: unconjugated

Histone H3 Mouse Monoclonal Antibody [6-A7]

Application: WB,IHC-P,IF-Tissue,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K23) Recombinant Rabbit Monoclonal Antibody [JE46-39]

Application: WB,IF-Cell

Reactivity: Human,Mouse

Conjugate: unconjugated

Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody [JU43-26]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,SNAP-ChIP,CUT&Tag-seq

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K9) Recombinant Rabbit Monoclonal Antibody [PSH04-47] - ChIP Grade

Application: WB,IF-Cell,IHC-P,IF-Tissue,FC,ChIP,Dot Blot,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (mono methyl K36) Recombinant Rabbit Monoclonal Antibody [SR04-20]

Application: WB,IF-Cell,IF-Tissue,ChIP

Reactivity: Human,Mouse

Conjugate: unconjugated

Histone H3 (acetyl K56) Recombinant Rabbit Monoclonal Antibody [SU30-10]

Application: WB,IF-Cell,IF-Tissue,IHC-P,ChIP,CUT&Tag-seq

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Conjugate: unconjugated

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