TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610425
TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric) - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within human TDP43 aa 365-414.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue, IF-Cell
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) lysate, HEK-293 (Human embryonic kidney cell) lysate, SH-SY5Y (Human neuroblastoma cell) lysate, K-562 (Human chronic myelogenous leukemia cell) lysate, C2C12 (Mouse myoblast) lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate, Mouse brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH09-14
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
TDP-43 is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. TDP-43 has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recent work has characterized the transcriptome-wide binding sites revealing that thousands of RNAs are bound by TDP-43 in neurons. TDP-43 was originally identified as a transcriptional repressor that binds to chromosomally integrated trans-activation response element (TAR) DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene and the apoA-II gene. In spinal motor neurons TDP-43 has also been shown in humans to be a low molecular weight neurofilament (hNFL) mRNA-binding protein. It has also shown to be a neuronal activity response factor in the dendrites of hippocampal neurons suggesting possible roles in regulating mRNA stability, transport and local translation in neurons. It has been demonstrated that zinc ions are able to induce aggregation of endogenous TDP-43 in cells. Moreover, zinc could bind to RNA binding domain of TDP-43 and induce the formation of amyloid-like aggregates in vitro.
Background References
1. McMillan M et al. RNA methylation influences TDP43 binding and disease pathogenesis in models of amyotrophic lateral sclerosis and frontotemporal dementia. Mol Cell. 2023 Jan
2. Corbet GA et al. TDP43 ribonucleoprotein granules: physiologic function to pathologic aggregates. RNA Biol. 2021 Oct
Subcellular Location
Cytoplasm, Mitochondrion, Nucleus.
Synonyms
ALS10 antibody
OTTHUMP00000002171 antibody
OTTHUMP00000002172 antibody
OTTHUMP00000002173 antibody
TADBP_HUMAN antibody
TAR DNA binding protein 43 antibody
TAR DNA binding protein antibody
TAR DNA-binding protein 43 antibody
TARDBP antibody
TDP 43 antibody
ExpandImages
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This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Western blot analysis of TDP43 on different lysates with Rabbit anti-TDP43 antibody (HA601627) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) lysate
Lane 2: HEK-293 (Human embryonic kidney cell) lysate
Lane 3: SH-SY5Y (Human neuroblastoma cell) lysate
Lane 4: K-562 (Human chronic myelogenous leukemia cell) lysate
Lane 5: C2C12 (Mouse myoblast) lysate
Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate
Lane 7: Mouse brain tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601627, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 45 kDa
Observed band size: 45 kDa -
This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601627, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601627, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601627, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601627, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601627, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: HeLa (Human cervix adenocarcinoma epithelial cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601627, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
This data was developed using HA601627, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: C2C12 (Mouse myoblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601627, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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