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TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric)

Usd: 385

Specification

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Catalog# HA601627

TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric)

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within human TDP43 aa 365-414.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, IF-Cell

Molecular Weight

Predicted band size: 45 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) lysate, HEK-293 (Human embryonic kidney cell) lysate, SH-SY5Y (Human neuroblastoma cell) lysate, K-562 (Human chronic myelogenous leukemia cell) lysate, C2C12 (Mouse myoblast) lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate, Mouse brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH09-14

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500-1:2,000

  • IF-Tissue

  • 1:500

  • IF-Cell

  • 1:50

Target

Function

TDP-43 is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. TDP-43 has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recent work has characterized the transcriptome-wide binding sites revealing that thousands of RNAs are bound by TDP-43 in neurons. TDP-43 was originally identified as a transcriptional repressor that binds to chromosomally integrated trans-activation response element (TAR) DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene and the apoA-II gene. In spinal motor neurons TDP-43 has also been shown in humans to be a low molecular weight neurofilament (hNFL) mRNA-binding protein. It has also shown to be a neuronal activity response factor in the dendrites of hippocampal neurons suggesting possible roles in regulating mRNA stability, transport and local translation in neurons. It has been demonstrated that zinc ions are able to induce aggregation of endogenous TDP-43 in cells. Moreover, zinc could bind to RNA binding domain of TDP-43 and induce the formation of amyloid-like aggregates in vitro.

Background References

1. McMillan M et al. RNA methylation influences TDP43 binding and disease pathogenesis in models of amyotrophic lateral sclerosis and frontotemporal dementia. Mol Cell. 2023 Jan

2. Corbet GA et al. TDP43 ribonucleoprotein granules: physiologic function to pathologic aggregates. RNA Biol. 2021 Oct

Subcellular Location

Cytoplasm, Mitochondrion, Nucleus.

UNIPROT

SwissProt: Q13148 Human

SwissProt: Q921F2 Mouse

Entrez Gene: 298648 Rat

Synonyms

ALS10 antibody

OTTHUMP00000002171 antibody

OTTHUMP00000002172 antibody

OTTHUMP00000002173 antibody

TADBP_HUMAN antibody

TAR DNA binding protein 43 antibody

TAR DNA binding protein antibody

TAR DNA-binding protein 43 antibody

TARDBP antibody

TDP 43 antibody

Expand

Images

  • Western blot analysis of TDP43 on different lysates with Rat anti-TDP43 antibody (<a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) lysate<br />Lane 2: HEK-293 (Human embryonic kidney cell) lysate<br />Lane 3: SH-SY5Y (Human neuroblastoma cell) lysate<br />Lane 4: K-562 (Human chronic myelogenous leukemia cell) lysate<br />Lane 5: C2C12 (Mouse myoblast) lysate<br />Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate<br />Lane 7: Mouse brain tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rat IgG-HRP (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa

    Western blot analysis of TDP43 on different lysates with Rat anti-TDP43 antibody (HA601627) at 1/2,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) lysate
    Lane 2: HEK-293 (Human embryonic kidney cell) lysate
    Lane 3: SH-SY5Y (Human neuroblastoma cell) lysate
    Lane 4: K-562 (Human chronic myelogenous leukemia cell) lysate
    Lane 5: C2C12 (Mouse myoblast) lysate
    Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate
    Lane 7: Mouse brain tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601627, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601627, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601627, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601627, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA601627, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Rat <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA601627, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: HeLa (Human cervix adenocarcinoma epithelial cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/50, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: HeLa (Human cervix adenocarcinoma epithelial cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA601627, 1/50, overnight at 4°C.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
    Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Mouse <br />Sample: C2C12 (Mouse myoblast) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA601627" style="font-weight: bold;text-decoration: underline;">HA601627</a>, 1/50, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: C2C12 (Mouse myoblast)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA601627, 1/50, overnight at 4°C.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
    Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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