iFluor™ 488 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69] (HA720217F)
Catalog# HA720217F
Rabbit Monoclonal to EpCAM
Tested for
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IF-Cell
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 488
Immunocytochemistry analysis of HT-29 cells labeling EpCAM with Rabbit anti-EpCAM antibody (HA720217F) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EpCAM antibody (HA720217F, iFluor™ 488) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EpCAM antibody (HA720217F, iFluor™ 488) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.