iFluor™ 488 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
Catalog# HA720217F
iFluor™ 488 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
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IF-Cell
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Human
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide.
Species Reactivity
Human
Validated Applications
IF-Cell
Molecular Weight
Predicted band size: 35 kDa
Positive Control
HT-29.
Conjugation
iFluor™ 488
Clone Number
PS01-69
Reactivity Data
| IF-Cell | |
|---|---|
| Human |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:1,000
Target
Function
EPCAM is a carcinoma-associated antigen and belongs to a family which includes at least 2 type I membrane proteins. The EPCAM protein has a role in embryonic stem cells proliferation and differentiation. EPCAM is used as a target for immunotherapy treatment of human carcinomas. EPCAM is expressed on most normal epithelial cells and gastrointestinal carcinomas and acts as a homotypic calcium-independent cell adhesion molecule. Epithelial cell adhesion molecules (EPCAM) can act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for supplying immunological barrier as a first line of defense against mucosal infection. EPCAM gene mutations result in congenital tufting enteropathy.
Background References
1. Li D et al. EpCAM-targeting CAR-T cell immunotherapy is safe and efficacious for epithelial tumors. Sci Adv. 2023 Dec
2. Li F et al. EpCAM-Targeting Aptamer Radiotracer for Tumor-Specific PET Imaging. Bioconjug Chem. 2021 Jun
Subcellular Location
Lateral cell membrane, Cell junction.
UNIPROT
Synonyms
17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody
AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
ExpandImages
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Immunocytochemistry analysis of HT-29 cells labeling EpCAM with Rabbit anti-EpCAM antibody (HA720217F) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EpCAM antibody (HA720217F, iFluor™ 488) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Tumor Cell-Expressed Herpesvirus Entry Mediator Regulates Proliferation and Adaptive Immunity in Ovarian Cancer
Journal: Immunity Inflammation And Disease
DOI: 10.1002/iid3.70175
IF: 3.1
Application: IF-tissue
Reactivity: Human
Publish date: 2025 Mar
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