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Phospho-Histone H3 (T3)
Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

DATA SHEET
MSDS
COA

Usd: 385

Specification

100μl

Quantity

Bulk Order Quote | Customization Request

Catalog# HA721796

Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Application

WB
IHC-P
IF-Cell
FC

Reactivity

Human
Mouse

Predicted reactivity

Predicted species support after-sales service

Conjugation

unconjugated

Overview

Product Name

Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr3 of Human Histone H3.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IF-Cell, FC

Molecular Weight

Predicted band size: 15 kDa

Positive Control

HeLa treated with 20% FBS and 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 20% PBS and 100nM Calyculin A for 30 minutes cell lysate, mouse breast tissue, mouse skin tissue, HeLa.

Conjugation

unconjugated

Clone Number

JE42-48

RRID

AB_3072908

Product Features

Form

Liquid

Concentration

Lot Concentration Lookup

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:1,000

IHC-P
1:200-1:1,000

IF-Cell
1:10,000-1:20,000

FC
1:1,000

Target

Function

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Background References

1. Strobino M. et. al. Loss of histone H3.3 results in DNA replication defects and altered origin dynamics in C. elegans. Genome Res. 2020 Dec

2. Ince AT. et. al. The relationship of Serum Histone H3.3 and H4 with chronic Hepatitis B. J Pak Med Assoc. 2020 Sep

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: P68431 Human

SwissProt: P68433 Mouse

SwissProt: Q6LED0 Rat

Synonyms

HIST1 cluster, H3J antibody

Histone gene cluster 1, H3 histone family, member E antibody

Histone gene cluster 1, H3G antibody

H3 histone family, member J antibody

HIST1 cluster, H3E antibody

HIST1 cluster, H3I antibody

Histone gene cluster 1, H3C antibody

FLJ92264 antibody

H3 histone family, member A antibody

H3 histone family, member B antibody

Expand

HIST1 cluster, H3J antibody

Histone gene cluster 1, H3 histone family, member E antibody

Histone gene cluster 1, H3G antibody

H3 histone family, member J antibody

HIST1 cluster, H3E antibody

HIST1 cluster, H3I antibody

Histone gene cluster 1, H3C antibody

FLJ92264 antibody

H3 histone family, member A antibody

H3 histone family, member B antibody

H3 histone family, member C antibody

H3 histone family, member D antibody

H3 histone family, member F antibody

H3 histone family, member H antibody

H3 histone family, member I antibody

H3 histone family, member K antibody

H3 histone family, member L antibody

H3 histone family, member T antibody

H3 histone, family 3A antibody

H3.1 antibody

H3.3A antibody

H3/a antibody

H3/b antibody

H3/c antibody

H3/d antibody

h3/f antibody

H3/h antibody

H3/i antibody

H3/j antibody

H3/k antibody

H3/l antibody

H3/t antibody

H31_HUMAN antibody

H3F1K antibody

H3F3 antibody

H3F3A antibody

H3FA antibody

H3FB antibody

H3FC antibody

H3FD antibody

H3FF antibody

H3FH antibody

H3FI antibody

H3FJ antibody

H3FK antibody

H3FL antibody

HIST1 cluster, H3A antibody

HIST1 cluster, H3B antibody

HIST1 cluster, H3C antibody

HIST1 cluster, H3D antibody

HIST1 cluster, H3F antibody

HIST1 cluster, H3G antibody

HIST1 cluster, H3H antibody

HIST1H3A antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

HIST1H3E antibody

HIST1H3F antibody

HIST1H3G antibody

HIST1H3H antibody

HIST1H3I antibody

HIST1H3J antibody

HIST3H3 antibody

Histone 1, H3a antibody

Histone 1, H3b antibody

Histone 1, H3c antibody

Histone 1, H3d antibody

Histone 1, H3e antibody

Histone 1, H3f antibody

Histone 1, H3g antibody

Histone 1, H3h antibody

Histone 1, H3i antibody

Histone 1, H3j antibody

histone 3, H3 antibody

histone cluster 1 H3 family member a antibody

histone cluster 1 H3 family member b antibody

histone cluster 1 H3 family member c antibody

histone cluster 1 H3 family member d antibody

histone cluster 1 H3 family member e antibody

histone cluster 1 H3 family member f antibody

histone cluster 1 H3 family member g antibody

histone cluster 1 H3 family member h antibody

histone cluster 1 H3 family member i antibody

histone cluster 1 H3 family member j antibody

Histone cluster 1, H3a antibody

Histone cluster 1, H3b antibody

Histone cluster 1, H3c antibody

Histone cluster 1, H3d antibody

Histone cluster 1, H3e antibody

Histone cluster 1, H3f antibody

Histone cluster 1, H3g antibody

Histone cluster 1, H3i antibody

Histone cluster 1, H3j antibody

Histone gene cluster 1, H3 histone family, member A antibody

Histone gene cluster 1, H3 histone family, member B antibody

Histone gene cluster 1, H3 histone family, member C antibody

Histone gene cluster 1, H3 histone family, member D antibody

Histone gene cluster 1, H3 histone family, member F antibody

Histone gene cluster 1, H3 histone family, member G antibody

Histone gene cluster 1, H3 histone family, member H antibody

Histone gene cluster 1, H3 histone family, member I antibody

Histone gene cluster 1, H3 histone family, member J antibody

Histone gene cluster 1, H3A antibody

Histone gene cluster 1, H3B antibody

Histone gene cluster 1, H3D antibody

Histone gene cluster 1, H3E antibody

Histone gene cluster 1, H3F antibody

Histone gene cluster 1, H3H antibody

Histone gene cluster 1, H3I antibody

Histone gene cluster 1, H3J antibody

Histone H 3 antibody

Histone H3.1 antibody

histone H3.1t antibody

Histone H3.2 antibody

Histone H3/a antibody

Histone H3/b antibody

Histone H3/c antibody

Histone H3/d antibody

Histone H3/f antibody

Histone H3/h antibody

Histone H3/i antibody

Histone H3/j antibody

Histone H3/k antibody

Histone H3/l antibody

Histone H3/m antibody

Histone H3/o antibody

Collapse

Images

☑ Cell treatment (CT)

Western blot analysis of Phospho-Histone H3 (T3) on different lysates with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 20% FBS and 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 20% PBS and 100nM Calyculin A for 30 minutes cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 25 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721796) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721796) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721796) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of HeLa cells labeling Phospho-Histone H3 (T3) with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/10,000 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of HeLa cells untreated (left) / treated with 20% FBS and 100nM Calyculin A for 30 minutes (right) labeling Phospho-Histone H3 (T3).

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721796, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Usd: 385

Specification

Overview

Product Name

Antibody Type

Immunogen

Species Reactivity

Validated Applications

Molecular Weight

Positive Control

Conjugation

Clone Number

RRID

Product Features

Form

Concentration

Storage Instructions

Storage Buffer

Isotype

Purification Method

Application Dilution

Target

Function

Background References

Subcellular Location

UNIPROT

Synonyms

Images

Products with the same target and pathway

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Application: WB,IF-Cell,IF-Tissue,IHC-P,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 Rabbit Polyclonal Antibody

Application: WB

Reactivity: Zebrafish,Human,Mouse

Conjugate: unconjugated

Histone H3 Mouse Monoclonal Antibody [6-A7]

Application: WB,IHC-P,IF-Tissue,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K23) Recombinant Rabbit Monoclonal Antibody [JE46-39]

Application: WB,IF-Cell

Reactivity: Human,Mouse

Conjugate: unconjugated

Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody [JU43-26]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,SNAP-ChIP,CUT&Tag-seq

Reactivity: Human,Mouse,Rat

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