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Histone H3 (citrulline R2) Recombinant Rabbit Monoclonal Antibody [PSH23-25]

Usd: 385

Specification

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Catalog# HA724360

Histone H3 (citrulline R2) Recombinant Rabbit Monoclonal Antibody [PSH23-25]

Application

  • WB

  • IP

Reactivity

  • Human

Predicted reactivity

  • Mouse

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

Histone H3 (citrulline R2) Recombinant Rabbit Monoclonal Antibody [PSH23-25]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 15 kDa

Positive Control

293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours.

Conjugation

unconjugated

Clone Number

PSH23-25

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Background References

1. Ouyang W et al. A citrullinated histone H3 monoclonal antibody for immune modulation in sepsis. Nat Commun. 2025 Aug

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: P68431 Human

Synonyms

H3FA

HIST1H3A

H3C2

H3FL

HIST1H3B

H3C3

H3FC HIST1H3C

H3C4

H3FB

HIST1H3D

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Histone H3 (citrulline R2) on different lysates with Rabbit anti-Histone H3 (citrulline R2) antibody (<a href="/products/HA724360" style="font-weight: bold;text-decoration: underline;">HA724360</a>) at 1/2,000 dilution. <br /> <br />Lane 1: 293T (Human embryonic kidney cells) cells transfected with PADI4 <br />Lane 2: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 4 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724360" style="font-weight: bold;text-decoration: underline;">HA724360</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 15 kDa <br />Observed band size: 15 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of Histone H3 (citrulline R2) on different lysates with Rabbit anti-Histone H3 (citrulline R2) antibody (HA724360) at 1/2,000 dilution.

    Lane 1: 293T (Human embryonic kidney cells) cells transfected with PADI4
    Lane 2: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 4 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724360, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunoprecipitation (IP) <br /> <br />Histone H3 (citrulline R2) was immunoprecipitated in 0.2 mg 293T (Human embryonic kidney cells) cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate with <a href="/products/HA724360" style="font-weight: bold;text-decoration: underline;">HA724360</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724360" style="font-weight: bold;text-decoration: underline;">HA724360</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. <br /> <br />Lane 1: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate (input) <br />Lane 2: <a href="/products/HA724360" style="font-weight: bold;text-decoration: underline;">HA724360</a> IP in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate <br />Lane 3: Rabbit IgG instead of <a href="/products/HA724360" style="font-weight: bold;text-decoration: underline;">HA724360</a> in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate <br /> <br />Exposure time: 2 minutes <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Primary dilution: <a href="/products/HA724360" style="font-weight: bold;text-decoration: underline;">HA724360</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature <br /> <br />Predicted band size: 15 kDa <br />Observed band size: 15 kDa

    ☑ Cell treatment (CT)

    Immunoprecipitation (IP)

    Histone H3 (citrulline R2) was immunoprecipitated in 0.2 mg 293T (Human embryonic kidney cells) cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate with HA724360 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724360 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate (input)
    Lane 2: HA724360 IP in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate
    Lane 3: Rabbit IgG instead of HA724360 in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate

    Exposure time: 2 minutes
    Blocking: 5% NFDM/TBST, 1 hour at room temperature

    Primary dilution: HA724360, 1/1,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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