CD11b Mouse Monoclonal Antibody [A2-9] (M1211-6)
Catalog# M1211-6
Mouse Monoclonal to CD11b
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: TF-1 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
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