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CD11b Mouse Monoclonal Antibody [A2-9]

Usd: 350

Specification

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Catalog# M1211-6

CD11b Mouse Monoclonal Antibody [A2-9]

Application

  • WB

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD11b Mouse Monoclonal Antibody [A2-9]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human CD11b 500-750 aa.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC, IF-Tissue

Molecular Weight

Predicted band size: 127 kDa

Positive Control

TF-1 cell lysate, human tonsil tissue, human spleen tissue, Raji.

Conjugation

unconjugated

Clone Number

A2-9

RRID

AB_3073051

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:200

  • IF-Tissue

  • 1:200

Target

Function

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Background References

1. "JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration." Zen K., Babbin B.A., Liu Y., Whelan J.B., Nusrat A., Parkos C.A. Mol. Biol. Cell 15:3926-3937(2004)

2. Nath S.K., Han S "A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus."., Kim-Howard X., Kelly J.A., Viswanathan P., Gilkeson G.S., Chen W., Zhu C., McEver R.P., Kimberly R.P., Alarcon-Riquelme M.E., Vyse T.J., Li Q.-Z., Wakeland E.K., Merrill J.T., James J.A., Kaufman K.M., Guthridge J.M., Harley J.B. Nat. Genet. 40:152-154(2008)

Sequence Similarity

Belongs to the integrin alpha chain family.

Tissue Specificity

Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).

Subcellular Location

Membrane.

UNIPROT

SwissProt: P11215 Human

Synonyms

antigen CD11b (p170) antibody

Antigen CD11b p170 antibody

CD11 antigen like family member B antibody

CD11 antigen-like family member B antibody

CD11b antibody

CD11b/CD18 antibody

CD49d antibody

Cell surface glycoprotein MAC-1 subunit alpha antibody

Complement component 3 receptor 3 subunit antibody

Complement Component Receptor 3 Alpha antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />Lane 1: TF-1 cell lysate<br />Lane 2: Jurkat cell lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 127 kDa<br />Observed band size: 170 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    Lane 1: TF-1 cell lysate
    Lane 2: Jurkat cell lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 127 kDa
    Observed band size: 170 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: Spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: Spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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