Rat CCL2/MCP-1 enzyme-linked immunoassay kit
Catalog# ER0016
Rat CCL2/MCP-1 enzyme-linked immunoassay kit
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ELISA
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Rat
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15.63 ~1000 pg/ml
Overview
Product Name
Rat CCL2/MCP-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.82 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0137 | 0.0142 | 0.0140 | |
| 15.63 | 0.0503 | 0.0500 | 0.0502 | 0.0366 |
| 31.25 | 0.0978 | 0.0987 | 0.0983 | 0.0841 |
| 62.50 | 0.1952 | 0.2032 | 0.1992 | 0.1815 |
| 125.00 | 0.4078 | 0.4140 | 0.4109 | 0.3941 |
| 250.00 | 0.8651 | 0.8468 | 0.8560 | 0.8514 |
| 500.00 | 1.7020 | 1.6940 | 1.6980 | 1.6883 |
| 1000.00 | 3.1350 | 3.2230 | 3.1790 | 3.1213 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 20.2 | 134.6 | 283.4 | 30.3 | 161.8 | 287.4 |
| Standard Deviation | 1.5 | 10.4 | 20.6 | 1.8 | 6.5 | 8.6 |
| Coefficient of Variation (%) | 7.6 | 7.7 | 7.3 | 5.9 | 4.0 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat CCL2/MCP-1 into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 107% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4475.15-7615.38 | 100 | 6120.63 |
Serum/Plasma– Thirty samples from apparently healthy rats were evaluated for the presence of rat CCL2/MCP-1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃