Rat CRP/C-Reactive Protein enzyme-linked immunoassay kit(one step)
Catalog# ERY0009
Rat CRP/C-Reactive Protein enzyme-linked immunoassay kit(one step)
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sELISA
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Rat
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0.03~20 ng/ml
Overview
Product Name
Rat CRP/C-Reactive Protein enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
2.9 pg/ml (50 μl)；42.15 pg/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0109 | 0.0107 | 0.0108 | |
| 0.03 | 0.0173 | 0.0170 | 0.0172 | 0.0064 |
| 0.08 | 0.0294 | 0.0284 | 0.0289 | 0.0181 |
| 0.25 | 0.0703 | 0.0708 | 0.0706 | 0.0598 |
| 0.74 | 0.1966 | 0.1900 | 0.1933 | 0.1825 |
| 2.22 | 0.5389 | 0.5387 | 0.5388 | 0.5280 |
| 6.67 | 1.4830 | 1.5790 | 1.5310 | 1.5202 |
| 20.00 | 3.7570 | 3.7950 | 3.7760 | 3.7652 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 0.4 | 2.1 | 6.4 | 0.4 | 2.4 | 6.9 |
| Standard Deviation | 0.0 | 0.1 | 0.3 | 0.0 | 0.1 | 0.2 |
| Coefficient of Variation (%) | 5.7 | 5.2 | 4.9 | 5.9 | 5.4 | 3.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Rat CRP into health Rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 120% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.22-3.51 | 100 | 2.41 |
Serum/Plasma – Thirty samples from apparently healthy rats were evaluated for the presence of CRP in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Pentraxin 1, short
C reactive protein
C reactive protein pentraxin related
C-reactive protein(1-205)
CRP
CRP_HUMAN
MGC88244
Pentraxin 1
PTX 1
PTX1
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