Rat IL-5 enzyme-linked immunoassay kit
Catalog# ER0014
Rat IL-5 enzyme-linked immunoassay kit
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ELISA
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Rat
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0.69~500 pg/ml
Overview
Product Name
Rat IL-5 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.14 pg/ml (50 μl)；0.69 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0146 | 0.0141 | 0.0144 | |
| 0.69 | 0.0223 | 0.0231 | 0.0227 | 0.0084 |
| 2.06 | 0.0396 | 0.0367 | 0.0382 | 0.0238 |
| 6.17 | 0.0784 | 0.0810 | 0.0797 | 0.0654 |
| 18.52 | 0.2175 | 0.2006 | 0.2091 | 0.1947 |
| 55.56 | 0.5625 | 0.5697 | 0.5661 | 0.5518 |
| 166.67 | 1.5290 | 1.5880 | 1.5585 | 1.5442 |
| 500.00 | 3.5670 | 3.6160 | 3.5915 | 3.5772 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 10.6 | 55.6 | 169.9 | 11.4 | 55.2 | 171.9 |
| Standard Deviation | 0.5 | 2.2 | 8.0 | 0.2 | 1.5 | 3.6 |
| Coefficient of Variation (%) | 4.3 | 4.0 | 4.7 | 1.5 | 2.8 | 2.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat IL-5 into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.06-0.69 | 100 | 0.33 |
Serum/Plasma – Thirty samples from apparently healthy rats were evaluated in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃