Rat TIMP1 enzyme-linked immunoassay kit
Catalog# ER0017
Rat TIMP1 enzyme-linked immunoassay kit
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ELISA
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Rat
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1.37~1000 pg/ml
Overview
Product Name
Rat TIMP1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.26 pg/ml (50 μl)；0.65 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0080 | 0.0098 | 0.0089 | |
| 1.37 | 0.0155 | 0.0154 | 0.0155 | 0.0066 |
| 4.12 | 0.0253 | 0.0271 | 0.0262 | 0.0173 |
| 12.35 | 0.0639 | 0.0646 | 0.0643 | 0.0554 |
| 37.04 | 0.1718 | 0.1772 | 0.1745 | 0.1656 |
| 111.11 | 0.5037 | 0.5181 | 0.5109 | 0.5020 |
| 333.33 | 1.4100 | 1.4330 | 1.4215 | 1.4126 |
| 1000.00 | 3.3520 | 3.3100 | 3.3310 | 3.3221 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.7 | 96.0 | 299.1 | 21.6 | 103.1 | 309.4 |
| Standard Deviation | 0.7 | 3.8 | 11.0 | 0.7 | 2.2 | 12.3 |
| Coefficient of Variation (%) | 3.7 | 4.0 | 3.7 | 3.2 | 2.2 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat TIMP1 into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 97%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.83-8.02 | 100 | 6.25 |
Serum/Plasma– Thirty samples from apparently healthy rats were evaluated for the presence of rat TIMP1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃