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CD11c Recombinant Rabbit Monoclonal Antibody

Usd: 385

Specification

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Catalog# IRS001

CD11c Recombinant Rabbit Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD11c Recombinant Rabbit Monoclonal Antibody

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD11c aa 1,114-1,163 / 1,163 (Cytoplasmic).

Species Reactivity

Human

Validated Applications

mIHC

Positive Control

Human cholangiocarcinoma tissue, human tonsil tissue, human colon tissue, human kidney tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.

Target

Function

Integrin αX (CD11C, leukocyte surface antigen p150,95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with β2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin αX/β2 shares similar properties of the αM/β2 integrin in mediating adherence of neutrophils and monocytes to stimulated endothelial cells, and in phagocytosis of complement coated particles. Abnormal expression of Integrin αX is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Proteins and DNA elements that influence transcription of Integrin αX include Sp1 and Sp1-like factors, AP-1 family, C/EBP, Oct-2 and PU.1. Integrin αX is present on monocyte derivative dendritic cells (DCs), macrophages and NK cells. Upon activation, DCs present in skin (Langerhans cells), lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B cells to initiate and shape the immune response.

Background References

1. Morandi F et al. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol 192:2634-42 (2014).

2. Svensson MN et al. Fms-like tyrosine kinase 3 ligand controls formation of regulatory T cells in autoimmune arthritis. PLoS One 8:e54884 (2013).

Sequence Similarity

Belongs to the integrin alpha chain family.

Tissue Specificity

Predominantly expressed in monocytes and granulocytes.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P20702 Human

Synonyms

95 alpha chain antibody

95 antibody

CD 11c antibody

CD11 antigen-like family member C antibody

CD11c antibody

CD11c antigen antibody

Complement component 3 receptor 4 subunit antibody

CR4 antibody

Integrin alpha X antibody

Integrin alpha X chain antibody

Expand

Images

  • mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), CD8 and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (IRS001), CD20 (IRS003), CD8 and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), CD38 (<a href="/products/IRS006" style="font-weight: bold;text-decoration: underline;">IRS006</a>), CD8 and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (IRS001), CD68 (IRS004), CD20 (IRS003), CD38 (IRS006), CD8 and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (<a href="/products/IRS005" style="font-weight: bold;text-decoration: underline;">IRS005</a>), FOXP3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD38 (<a href="/products/IRS006" style="font-weight: bold;text-decoration: underline;">IRS006</a>), CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), PD-L1, CD163 (<a href="/products/IRS002" style="font-weight: bold;text-decoration: underline;">IRS002</a>) and CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (IRS005), FOXP3, CD68 (IRS004), CD38 (IRS006), CD11c (IRS001), PD-L1, CD163 (IRS002) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Ki67 (<a href="/products/IRS036" style="font-weight: bold;text-decoration: underline;">IRS036</a>), CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), PD-1 and CD8 (<a href="/products/IRS007" style="font-weight: bold;text-decoration: underline;">IRS007</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Ki67 (IRS036), CD11c (IRS001), PD-1 and CD8 (IRS007) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD38 (<a href="/products/IRS006" style="font-weight: bold;text-decoration: underline;">IRS006</a>), CD57 (<a href="/products/IRS027" style="font-weight: bold;text-decoration: underline;">IRS027</a>), CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), Tryptase (<a href="/products/IRS041" style="font-weight: bold;text-decoration: underline;">IRS041</a>) and CD45 (<a href="/products/IRS025" style="font-weight: bold;text-decoration: underline;">IRS025</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD38 (IRS006), CD57 (IRS027), CD11c (IRS001), Tryptase (IRS041) and CD45 (IRS025) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human craniopharyngioma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human craniopharyngioma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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