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CD20 Recombinant Rabbit Monoclonal Antibody

Usd: 385

Specification

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Catalog# IRS003

CD20 Recombinant Rabbit Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD20 Recombinant Rabbit Monoclonal Antibody

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD20 aa 210-297 (Cytoplasmic).

Species Reactivity

Human

Validated Applications

mIHC

Positive Control

Human tonsil tissue, human lung cancer with brain metastasis tissue, human cervical cancer tissue, human gastric cancer tissue, human small cell lung cancer tissue, human pancreatic cancer tissue, human lung tissue, human liver tissue, human spleen tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.

Target

Function

The CD20 antigen is a membrane-embedded, non-glycosylated phosphoprotein, 33-37 kDa. CD20 functions as a Ca2+-permeable cation channel, involved in the regulation of B-cell activation, proliferation and differentiation. CD20 appears on the surface of the pre-B lymphocyte between the time of light chain rearrangement and expression of intact surface immunoglobulin and is lost just before terminal B-cell differentiation into plasma cells. CD20 is virtually specific for normal B-cells. A weak expression has been demonstrated in a subpopulation of T-cells, but not in any other cell type. CD20 is expressed in the large majority of cases of B-cell leukaemia/lymphoma. Early stage precursor B lymphoblastic leukaemia/lymphoma may be negative, and chronic lymphocytic leukaemia/small cell lymphoma may show a weak staining. Plasma cell neoplasms are as a rule CD20 negative. T-cell lymphomas are almost always negative, but CD20 has been demonstrated in few cases of various types of T-cell lymphoma. In Hodgkin lymphoma, the nodular lymphocyte-predominant subtype shows CD20 staining of L&H cells in most cases, while Reed-Sternberg cells in the other subtypes reveal CD20 positivity in about 40, albeit in a minority of neoplastic cells. Acute myeloid leukaemia is CD20 positive in few cases, while blastic transformation in chronic myeloid leukaemia is accompanied by CD20 positivity in about 30%. Thymoma may reveal CD20 positivity in a spindle cell component. In patients treated with retuximab (a humanized anti-CD20 antibody) for malignant B-cell lymphoma, the CD20 epitopes disappear (both in normal and neoplastic B-cells) as a result of down-modulation of CD20 m-RNA in the cells. This process is potentially reversible. Together with CD79a, CD20 is one of the most important markers for the identification of B-cell neoplasms as outlined above. Tonsil and appendix are appropriate controls: The mantle zone B-cells and the germinal centre B-cells must show a very strong staining reaction. No other cells should stain.

Background References

1. Jiang D. et. al. Pyruvate dehydrogenase kinase 4-mediated metabolic reprogramming is involved in rituximab resistance in diffuse large B-cell lymphoma by affecting the expression of MS4A1/CD20. Cancer Sci. 2021 Sep

2. Pavlasova G. et. al. The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy. Haematologica. 2020 Jun

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P11836 Human

Synonyms

APY antibody

ATOPY antibody

B lymphocyte antigen CD20 antibody

B Lymphocyte Cell Surface Antigen B1 antibody

B-lymphocyte antigen CD20 antibody

B-lymphocyte cell-surface antigen B1 antibody

B-lymphocyte surface antigen B1 antibody

B1 antibody

Bp 35 antibody

Bp35 antibody

Expand

Images

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (<a href="/products/IRS005" style="font-weight: bold;text-decoration: underline;">IRS005</a>), FOXP3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD38 (<a href="/products/IRS006" style="font-weight: bold;text-decoration: underline;">IRS006</a>), CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), PD-L1, CD163 (<a href="/products/IRS002" style="font-weight: bold;text-decoration: underline;">IRS002</a>) and CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (IRS005), FOXP3, CD68 (IRS004), CD38 (IRS006), CD11c (IRS001), PD-L1, CD163 (IRS002) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human lung cancer with brain metastasis tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>) and Ki67 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung cancer with brain metastasis tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD20 (IRS003), panCK (IRS010), CD68 (IRS004) and Ki67 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), FOXP3, CK19 (<a href="/products/IRS011" style="font-weight: bold;text-decoration: underline;">IRS011</a>), PD-L1, CD4 (<a href="/products/IRS005" style="font-weight: bold;text-decoration: underline;">IRS005</a>), CD163 (<a href="/products/IRS002" style="font-weight: bold;text-decoration: underline;">IRS002</a>) and CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), FOXP3, CK19 (IRS011), PD-L1, CD4 (IRS005), CD163 (IRS002) and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human colorectal cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD4 (<a href="/products/IRS005" style="font-weight: bold;text-decoration: underline;">IRS005</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human colorectal cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), CD68 (IRS004), CD4 (IRS005) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human small cell lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human small cell lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human pancreatic cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human pancreatic cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD56 (<a href="/products/IRS026" style="font-weight: bold;text-decoration: underline;">IRS026</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD163 (<a href="/products/IRS002" style="font-weight: bold;text-decoration: underline;">IRS002</a>), panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) and CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD56 (IRS026), CD68 (IRS004), CD163 (IRS002), panCK (IRS010) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human lung tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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