Human CD155/PVR(Poliovirus Receptor) DTSet enzyme-linked immunoassay kit

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Catalog# DSEH015505；DSEH015515

Human CD155/PVR(Poliovirus Receptor) DTSet enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Overview

Product Name

Human CD155/PVR(Poliovirus Receptor) DTSet enzyme-linked immunoassay kit

Product Type

DTSet Elisa Kit

pg/ml	O.D.		Average	Corrected
0.00	0.0277	0.0335	0.0306
312.50	0.0615	0.0580	0.0598	0.0292
625.00	0.1073	0.1096	0.1085	0.0779
1250.00	0.2368	0.2466	0.2417	0.2111
2500.00	0.6769	0.5968	0.6369	0.6063
5000.00	1.8730	1.7130	1.7930	1.7624
10000.00	3.8490	3.7014	3.7752	3.7446
20000.00	4.5993	4.6608	4.6301	4.5995

Experiment Type

Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)

Species Reactivity

Human

Detection Method

Colorimetric Method

Experiment Detection Duration

120min

Standard Curve Range

312.5pg/ml-20000pg/ml

Standard Curve Gradient

7 Points/3 Folds

Number of Incubations

Detectable Sample

Sample Loading Volume

50 μl

Product Features

Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
A development protocol is provided to guide further analysis optimization.
Detection can be customized according to your specific needs.
An economical alternative to a complete kit.

Kit Components

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin-HRP Conjugate

Other Required Materials

DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.

The components listed above can be purchased separately.

96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。

Target

UNIPROT

P15151

Synonyms

CD155

CD155 antigen

FLJ25946

HVED

mE4

NECL 5

Necl-5

Necl5

Nectin like 5

Nectin like protein 5

Expand

Function

CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules. Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin. While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains. The V-type domain mediates polio virus binding. Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60‑65 kDa soluble forms that show removal of the TM segment and surrounding amino acids. The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels. CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1. DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.

Human CD155/PVR(Poliovirus Receptor) DTSet enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Experiment Type

Species Reactivity

Detection Method

Experiment Detection Duration

Standard Curve Range

Standard Curve Gradient

Number of Incubations

Detectable Sample

Sample Loading Volume

Product Features

Kit Components

Other Required Materials

Target

UNIPROT

Synonyms

Function

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