Human Osteopontin/OPN DTSet enzyme-linked immunoassay kit

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Catalog# DSEH005705；DSEH005715

Human Osteopontin/OPN DTSet enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Overview

Product Name

Human Osteopontin/OPN DTSet enzyme-linked immunoassay kit

Product Type

DTSet Elisa Kit

pg/ml	O.D.		Average	Corrected
0.00	0.0157	0.0154	0.0156
62.50	0.0384	0.0375	0.0380	0.0224
125.00	0.0766	0.0737	0.0752	0.0596
250.00	0.1785	0.1750	0.1768	0.1612
500.00	0.4878	0.4852	0.4865	0.4710
1000.00	1.3640	1.2860	1.3250	1.3095
2000.00	2.9890	2.8330	2.9110	2.8955
4000.00	4.2053	4.3078	4.2566	4.2410

Experiment Type

Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)

Species Reactivity

Human

Detection Method

Colorimetric Method

Experiment Detection Duration

120min

Standard Curve Range

62.50 pg/ml-4000 pg/ml

Standard Curve Gradient

7点/3倍

Number of Incubations

Detectable Sample

Sample Loading Volume

50 μl

Product Features

Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
A development protocol is provided to guide further analysis optimization.
Detection can be customized according to your specific needs.
An economical alternative to a complete kit.

Kit Components

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin-HRP Conjugate

Other Required Materials

DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.

The components listed above can be purchased separately.

96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。

Target

UNIPROT

P10451

Synonyms

BNSP

Bone sialoprotein 1

BSP I

BSPI

Early T lymphocyte activation 1

ETA 1

ETA1

MGC110940

Nephropontin

OPN

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Human Osteopontin/OPN DTSet enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Experiment Type

Species Reactivity

Detection Method

Experiment Detection Duration

Standard Curve Range

Standard Curve Gradient

Number of Incubations

Detectable Sample

Sample Loading Volume

Product Features

Kit Components

Other Required Materials

Target

UNIPROT

Synonyms

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