Human IL-1RA/IL-1F3 DTSet enzyme-linked immunoassay kit

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Catalog# DSEH017705；DSEH017715

Human IL-1RA/IL-1F3 DTSet enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Overview

Product Name

Human IL-1RA/IL-1F3 DTSet enzyme-linked immunoassay kit

Product Type

DTSet Elisa Kit

pg/ml	O.D.		Average	Corrected
0.00	0.0704	0.0690	0.0697
31.25	0.3218	0.2896	0.3057	0.2360
62.50	0.4407	0.5047	0.4727	0.4030
125.00	0.7050	0.7112	0.7081	0.6384
250.00	1.2660	1.2780	1.2720	1.2023
500.00	2.2600	2.1290	2.1945	2.1248
1000.00	3.3460	2.9920	3.1690	3.0993
2000.00	4.0657	4.0810	4.0734	4.0037

Experiment Type

Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)

Species Reactivity

Human

Detection Method

Colorimetric Method

Experiment Detection Duration

120min

Standard Curve Range

31.25 pg/ml -2000 pg/ml

Standard Curve Gradient

7 Points/3 Folds

Number of Incubations

Detectable Sample

Sample Loading Volume

50 μl

Product Features

Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
A development protocol is provided to guide further analysis optimization.
Detection can be customized according to your specific needs.
An economical alternative to a complete kit.

Kit Components

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin-HRP Conjugate

Other Required Materials

DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.

The components listed above can be purchased separately.

96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。

Target

UNIPROT

SwissProt: P18510 Human

Synonyms

DIRA

F630041P17Rik

ICIL 1RA

ICIL-1RA

ICIL1RA

IL-1ra

IL-1ra3

IL-1RN

IL1 inhibitor

IL1F3

Expand

Function

Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2. Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity. It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations. IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response. Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region. Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component. Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells. A number of cell types express IL-1ra, including monocytes, Sertoli cells, hepatocytes, adipocytes, synovial fibroblasts, mast cells, pancreatic beta -cells, and intestinal epithelial cells. There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor. Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion. Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra.

Human IL-1RA/IL-1F3 DTSet enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Experiment Type

Species Reactivity

Detection Method

Experiment Detection Duration

Standard Curve Range

Standard Curve Gradient

Number of Incubations

Detectable Sample

Sample Loading Volume

Product Features

Kit Components

Other Required Materials

Target

UNIPROT

Synonyms

Function

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