Human IL-1RA/IL-1F3 DTSet enzyme-linked immunoassay kit
Catalog# DSEH017705；DSEH017715
Human IL-1RA/IL-1F3 DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Overview
Product Name
Human IL-1RA/IL-1F3 DTSet enzyme-linked immunoassay kit
Product Type
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0704 | 0.0690 | 0.0697 | |
| 31.25 | 0.3218 | 0.2896 | 0.3057 | 0.2360 |
| 62.50 | 0.4407 | 0.5047 | 0.4727 | 0.4030 |
| 125.00 | 0.7050 | 0.7112 | 0.7081 | 0.6384 |
| 250.00 | 1.2660 | 1.2780 | 1.2720 | 1.2023 |
| 500.00 | 2.2600 | 2.1290 | 2.1945 | 2.1248 |
| 1000.00 | 3.3460 | 2.9920 | 3.1690 | 3.0993 |
| 2000.00 | 4.0657 | 4.0810 | 4.0734 | 4.0037 |
Experiment Type
Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)
Species Reactivity
Human
Detection Method
Colorimetric Method
Experiment Detection Duration
120min
Standard Curve Range
31.25 pg/ml -2000 pg/ml
Standard Curve Gradient
7点/3倍
Number of Incubations
2
Detectable Sample
Sample Loading Volume
50 μl
Product Features
- Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
- A development protocol is provided to guide further analysis optimization.
- Detection can be customized according to your specific needs.
- An economical alternative to a complete kit.
Kit Components
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin-HRP Conjugate
Other Required Materials
DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.
The components listed above can be purchased separately.
- 96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
- Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
- Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
- Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
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