Human CD48 DTSet enzyme-linked immunoassay kit
Catalog# DSEH011905；DSEH011915
Human CD48 DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Overview
Product Name
Human CD48 DTSet enzyme-linked immunoassay kit
Product Type
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0196 | 0.0155 | 0.0176 | |
| 15.63 | 0.1035 | 0.1100 | 0.1068 | 0.0892 |
| 31.25 | 0.1959 | 0.1997 | 0.1978 | 0.1803 |
| 62.50 | 0.3659 | 0.3750 | 0.3705 | 0.3529 |
| 125.00 | 0.6445 | 0.7102 | 0.6774 | 0.6598 |
| 250.00 | 1.1900 | 1.2330 | 1.2115 | 1.1940 |
| 500.00 | 2.1300 | 2.2540 | 2.1920 | 2.1745 |
| 1000.00 | 3.5670 | 3.7450 | 3.6560 | 3.6385 |
Experiment Type
Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)
Species Reactivity
Human
Detection Method
Colorimetric Method
Experiment Detection Duration
120min
Standard Curve Range
15.63pg/ml-1000pg/ml
Standard Curve Gradient
7点/3倍
Number of Incubations
2
Detectable Sample
Sample Loading Volume
50 μl
Product Features
- Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
- A development protocol is provided to guide further analysis optimization.
- Detection can be customized according to your specific needs.
- An economical alternative to a complete kit.
Kit Components
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin-HRP Conjugate
Other Required Materials
DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.
The components listed above can be purchased separately.
- 96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
- Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
- Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
- Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
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