Human FGF-19 DTSet enzyme-linked immunoassay kit

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Catalog# DSEH014005；DSEH014015

Human FGF-19 DTSet enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Overview

Product Name

Human FGF-19 DTSet enzyme-linked immunoassay kit

Product Type

DTSet Elisa Kit

pg/ml	O.D.		Average	Corrected
0.00	0.0399	0.0366	0.0383
15.63	0.1331	0.1290	0.1311	0.0928
31.25	0.2248	0.2205	0.2227	0.1844
62.50	0.4076	0.4103	0.4090	0.3707
125.00	0.7020	0.7582	0.7301	0.6919
250.00	1.3920	1.4060	1.3990	1.3608
500.00	2.3510	2.5800	2.4655	2.4273
1000.00	3.8820	4.0277	3.9549	3.9166

Experiment Type

Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)

Species Reactivity

Human

Detection Method

Colorimetric Method

Experiment Detection Duration

120min

Standard Curve Range

15.63pg/ml-1000pg/ml

Standard Curve Gradient

7 Points/3 Folds

Number of Incubations

Detectable Sample

Sample Loading Volume

50 μl

Product Features

Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
A development protocol is provided to guide further analysis optimization.
Detection can be customized according to your specific needs.
An economical alternative to a complete kit.

Kit Components

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin-HRP Conjugate

Other Required Materials

DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.

The components listed above can be purchased separately.

96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。

Target

UNIPROT

O95750

Synonyms

FGF 19

FGF-19

FGF15

FGF19

FGF19_HUMAN

Fibroblast growth factor 15

Fibroblast growth factor 19

Function

The Fibroblast Growth Factor (FGF) family consists of at least 22 highly conserved proteins and are found in many species ranging from C. elegans and Drosophila to humans. FGFs are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. In general, FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. The activities of the FGF family are mediated by four receptors, FGF R1 through FGF R4. The receptors are transmembrane proteins with a tyrosine kinase domain and are thought to act as classical receptor tyrosine kinases. FGF R5/FGF RL1 has also been described, but lacks the kinase domain and signaling capability. Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites. It shares approximately 50% aa sequence identity with mouse and rat FGF-15 and 62% aa sequence identity with chicken FGF-19. Unlike most FGFs, which bind to and activate more than one FGF receptor, FGF-19 appears to bind only FGF R4. Receptor binding is capable of activating MAP Kinase and inducing the Prolactin promoter. FGF-19 has two putative disulfide bonds, one of which is conserved in the FGF family. Uniquely, it also has an extended loop structure that may affect heparan sulfate binding. The functional roles of FGF-19 continue to be elucidated and include developmental, metabolic, and tumorigenic activities. During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development. However, the mouse FGF-19 ortholog, FGF-15, is not required for this activity. FGF-19 is also expressed in the embryonic chicken eye where it may play a role in lens development. Questions regarding the functions of human FGF-19 have been addressed using ectopic expression. Transgenic over-expression of human FGF-19 in mice results in tumor formation, increased energy expenditure, decreased adiposity, and a resistance to weight gain in response to a high fat diet. Similar affects have been observed in mice treated intravenously with recombinant FGF-19.

Human FGF-19 DTSet enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Experiment Type

Species Reactivity

Detection Method

Experiment Detection Duration

Standard Curve Range

Standard Curve Gradient

Number of Incubations

Detectable Sample

Sample Loading Volume

Product Features

Kit Components

Other Required Materials

Target

UNIPROT

Synonyms

Function

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