Human IL-27 DTSet enzyme-linked immunoassay kit

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Catalog# DSEH009405；DSEH009415

Human IL-27 DTSet enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Overview

Product Name

Human IL-27 DTSet enzyme-linked immunoassay kit

Product Type

DTSet Elisa Kit

pg/ml	O.D.		Average	Corrected
0.00	0.0535	0.0543	0.0539
	13.72	0.0775	0.0785	0.0780
0.0780	41.15	0.1067	0.1056	0.1062
0.1062	123.46	0.1790	0.1789	0.1790
0.1790	370.37	0.3897	0.3746	0.3822
0.3822	1111.11	0.9672	0.9435	0.9554
0.9554	3333.33	2.2711	2.2609	2.2660
2.2660	10000.00	3.9974	3.8924	3.9449

Experiment Type

Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)

Species Reactivity

Human

Detection Method

Colorimetric Method

Experiment Detection Duration

120min

Standard Curve Range

13.72 pg/ml-10000 pg/ml

Standard Curve Gradient

7点/3倍

Number of Incubations

Detectable Sample

Sample Loading Volume

50 μl

Product Features

Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
A development protocol is provided to guide further analysis optimization.
Detection can be customized according to your specific needs.
An economical alternative to a complete kit.

Kit Components

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin-HRP Conjugate

Other Required Materials

DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.

The components listed above can be purchased separately.

96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。

Target

UNIPROT

Q8NEV9

Synonyms

EBI3

IL 27

IL 27 p28 subunit

IL 27A

IL 30

IL-27 subunit alpha

IL-27-A

IL27

IL27-A

IL27A

Expand

Human IL-27 DTSet enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Experiment Type

Species Reactivity

Detection Method

Experiment Detection Duration

Standard Curve Range

Standard Curve Gradient

Number of Incubations

Detectable Sample

Sample Loading Volume

Product Features

Kit Components

Other Required Materials

Target

UNIPROT

Synonyms

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